Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Amperometry: Overview

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

Controlled-Current Coulometry: Overview

Controlled-Current Coulometry: Overview

Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...

Electrogravimetric Analysis: Overview

Electrogravimetric Analysis: Overview

Electrogravimetric analysis measures the weight of an analyte deposited electrolytically onto a suitable working electrode. This method involves applying a potential to a pre-weighed electrode submerged in a solution, which results in the desired substance being deposited through reduction at the cathode or oxidation at the anode. The electrode's weight is recorded after deposition, and the difference in weight gives the analyte's weight in the solution.
To test the completeness of the...

Voltammetric Techniques: Pulse Voltammetry

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...

Interfacial Electrochemical Methods: Overview

Interfacial Electrochemical Methods: Overview

Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current...

Voltammetric Techniques: Linear-Scan (E vs Time)

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Dual-Barrel Carbon Fiber Microelectrode for Generator-Collector Experiments.

ACS omega·2025

Same author

Cost-effective quantification of uric acid using niobium oxide and graphene oxide-modified pencil-drawn electrodes on PVC substrates.

Analytical methods : advancing methods and applications·2024

Same author

A home-made nanoporous gold microsensor for lead(II) detection in seawater with high sensitivity and anti-interference properties.

Analytical methods : advancing methods and applications·2024

Same author

Film before aggregates: an <i>operando</i> GISAXS study on electrochemically assisted surfactant assembly.

Nanoscale·2024

Same author

Spatio-temporal detachment of homogeneous electron transfer in controlling selectivity in mediated organic electrosynthesis.

Faraday discussions·2023

Same author

Patterning (Electro)chemical Treatment-Free Electrodes with a 3D Printing Pen.

Analytical chemistry·2023

Search research articles

Related Experiment Video

Updated: Jun 14, 2025

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Published on: August 4, 2023

Calibration-Free Analysis with Chronoamperometry at Microelectrodes.

Valdomiro S Conceição¹, Douglas P M Saraiva¹, Guy Denuault²

¹Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo-USP, São Paulo, 05508-000, Brazil.

Analytical Chemistry

|September 3, 2024

Summary

This summary is machine-generated.

A novel electroanalytical method quantifies chemical concentrations using a single chronoamperometric curve, eliminating the need for calibration standards. This rapid technique offers a promising alternative for field analysis and autonomous sensors.

More Related Videos

Fabrication of Amperometric Electrodes

Fabrication of Amperometric Electrodes

Published on: May 4, 2009

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Published on: November 21, 2012

Related Experiment Videos

Last Updated: Jun 14, 2025

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Published on: August 4, 2023

Fabrication of Amperometric Electrodes

Fabrication of Amperometric Electrodes

Published on: May 4, 2009

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Published on: November 21, 2012

Area of Science:

Analytical Chemistry
Electrochemistry
Sensor Technology

Background:

Traditional electroanalytical methods often require calibration standards, facing limitations like matrix interference, instability, and cost.
There is a growing need for analytical techniques that are miniaturized, require less sample, and provide real-time field analysis.

Purpose of the Study:

To develop a new quantification method for chemical analysis that eliminates the need for calibration standards.
To demonstrate the feasibility of obtaining accurate analytical information from a single chronoamperometric experiment using a microelectrode.

Main Methods:

A novel quantification strategy based on a single chronoamperometric curve recorded with a microelectrode.
Exploration of various data treatments for determining unknown concentrations.
Investigation of experimental conditions, instrument parameters, and the impact of parallel reactions.

Main Results:

Satisfactory analytical information was obtained from a single, rapid chronoamperometric experiment.
The method successfully determined concentrations of ascorbic acid in food supplements and paracetamol in painkillers.
Results for ascorbic acid showed good agreement with coulometry (max deviation ~10.8%) and were validated in solutions of varying viscosity.

Conclusions:

The proposed calibration-free method offers a simplified and efficient approach to electroanalytical quantification.
The technique is suitable for determining individual or summed concentrations of multiple species.
This method holds potential for implementation in autonomous sensors for real-time field monitoring.