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Related Experiment Videos

Susceptibility testing with the sensititer breakpoint broth microdilution system.

G V Doern, A Dascal, M Keville

    Diagnostic Microbiology and Infectious Disease
    |May 1, 1985
    PubMed
    Summary
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    The Sensititer Breakpoint System (SBS) demonstrated high accuracy in determining antimicrobial susceptibility, comparable to standard disk diffusion methods for most bacteria. Minor discrepancies were noted, with specific issues for non-enterococcal streptococci against aminoglycosides.

    Area of Science:

    • Clinical Microbiology
    • Bacteriology
    • Antimicrobial Resistance

    Background:

    • Accurate antimicrobial susceptibility testing is crucial for effective treatment of bacterial infections.
    • Standardized disk diffusion is a common method, but newer automated systems offer potential advantages in speed and standardization.

    Purpose of the Study:

    • To evaluate the accuracy and concordance of the Sensititer Breakpoint System (SBS), a novel broth microdilution method, against a standardized disk diffusion procedure.
    • To identify any specific organism-antimicrobial combinations where the SBS may show significant discrepancies.

    Main Methods:

    • Determined antimicrobial susceptibility profiles for 318 bacterial isolates (255 gram-negative bacilli, 63 gram-positive cocci) using the SBS.
    • Compared SBS results with those from a standardized disk diffusion procedure across 4,414 organism-antimicrobial comparisons.

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  • Arbitrated major and very major discrepancies using a full-range minimum inhibitory concentration (MIC) procedure.

    • Main Results:

    • Overall concordance between SBS and disk diffusion was 88.1% (3,888/4,414).
    • Identified 9.6% minor, 1.0% major, and 1.3% very major discrepancies.
    • SBS results were confirmed in 53.4% of arbitrated major/very major discrepancies.
    • A notable exception was non-enterococcal streptococci against aminoglycosides, where disk diffusion results were favored due to potential issues with bacterial growth in SBS.

    Conclusions:

    • The SBS is a reliable method for antimicrobial susceptibility testing, demonstrating accuracy comparable to or exceeding standardized disk diffusion for most bacterial types.
    • The SBS shows high concordance with disk diffusion, with a low rate of major and very major discrepancies.
    • Further investigation into specific organism-growth medium interactions may be warranted for optimizing SBS performance in certain challenging cases.