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Updated: Jun 14, 2025

Tuning Degradation to Achieve Specific and Efficient Protein Depletion
Published on: July 20, 2019
The TATA-box binding protein-associated factor TAF12b facilitates the degradation of type B response regulators to
Liu-Ming Guo1, Jing Li2, Pan-Pan Qi2
1Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, China; Laboratory of Virology, Innovation Center of Chinese Medicine Crops, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Abstract:
Cytokinins (CKs) are one of the important classes of plant hormones essential for plant growth and development. TATA-box binding protein-associated factor 12b (TAF12b) is involved in CK signaling, but its molecular and biochemical mechanisms are not fully understood. In this study, TAF12b of Nicotiana benthamiana (NbTAF12b) was found to mediate the CK response by directly interacting with type B response regulators (B-RRs), positive regulators of CK signaling, and inhibiting their transcriptional activities. As a transcriptional co-factor, TAF12b specifically facilitated the proteasomal degradation of non-phosphorylated B-RRs by recruiting the KISS ME DEADLY family of F-box proteins. Such interactions between TAF12b and B-RRs also occur in other plant species. Genetic transformation experiments showed that overexpression of NbTAF12b attenuates the CK-hypersensitive phenotype conferred by NbRR1 overexpression. Taken together, these results suggest a conserved mechanism in which TAF12b negatively regulates CK responses by promoting 26S proteasome-mediated B-RR degradation in multiple plant species, providing novel insights into the regulatory network of CK signaling in plants.
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