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Related Experiment Video

Updated: Jun 14, 2025

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
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Improved Liver Intravital Microscopic Imaging Using a Film-Assisted Stabilization Method.

Libang Xu1, Xiaobing Feng1, Dazhi Wang2

  • 1School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

ACS Sensors
|September 4, 2024
PubMed
Summary

This study introduces liquid bridges to stabilize mouse livers during intravital microscopy (IVM). This novel vibration suppression technique significantly improves imaging quality for observing dynamic biological processes.

Keywords:
film-assisted vibration dampingintravital microscopyliposomes phagocytosisliquid bridgeliver IVM

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Area of Science:

  • Biomedical Engineering
  • Microscopy Techniques
  • Biophysics

Background:

  • Intravital microscopy (IVM) is crucial for studying dynamic biological processes in vivo.
  • Vibration from live animal physiology (respiration, heartbeat) poses a significant challenge to IVM image quality.
  • Existing methods for vibration suppression in IVM are often insufficient.

Purpose of the Study:

  • To investigate the vibration inhibition effect of liquid bridges on mouse liver during IVM.
  • To develop a novel vibration damping method for enhanced IVM.
  • To demonstrate the practical application of this technique in observing cellular processes.

Main Methods:

  • Studied friction characteristics of moist surfaces on mouse liver to understand liquid bridge formation.
  • Utilized fluorescence imaging to confirm the presence and nature of liquid bridges.
  • Developed a microscope-integrated device with adjustable polymer film tension to construct and stabilize liquid bridges.
  • Applied the method to liver IVM, observing liposome phagocytosis by Kupffer cells.

Main Results:

  • Confirmed the formation of microscale, nondestructive liquid bridges on the mouse liver surface.
  • Demonstrated that liquid bridges effectively stabilize the liver, significantly reducing vibration.
  • Achieved substantially improved image and video quality during IVM of liver processes.
  • Successfully observed liposome phagocytosis by Kupffer cells with enhanced clarity.

Conclusions:

  • Liquid bridges offer a feasible and effective solution for vibration suppression in intravital microscopy.
  • The developed technique enhances image quality, enabling better observation of dynamic biological events.
  • This soft, nondestructive vibration damping approach has potential applications beyond IVM, including precision instruments.