The CALERIE^™ Genomic Data Resource.

Area of Science:

• Gerontology
• Genomics
• Molecular Biology

Background:

• Caloric restriction (CR) is known to slow biological aging and extend lifespan in model organisms.
• The CALERIE-2 trial provided the first randomized, controlled human data on long-term CR in non-obese individuals.
• Understanding the molecular mechanisms of CR in humans is crucial for aging research.

Purpose of the Study:

• To generate and provide a comprehensive genomic dataset from the CALERIE-2 trial.
• To facilitate research into the molecular pathways and biological processes affected by CR in humans.
• To establish the first publicly accessible multi-omic, longitudinal data resource for a human aging intervention trial.

Main Methods:

• Generated whole-genome SNP genotypes from 218 participants.
• Collected longitudinal DNA methylation, mRNA, and small RNA data at three time points.
• Utilized blood, skeletal muscle, and adipose tissue samples (n=2327 total samples).

Main Results:

• Created a unique, multi-tissue, multi-omic, longitudinal dataset from a human CR trial.
• The CALERIE Genomic Data Resource is now publicly available via the Aging Research Biobank.
• This resource enables in-depth analysis of CR's molecular effects in humans.

Conclusions:

• The CALERIE Genomic Data Resource is a valuable asset for translational geroscience.
• This data can significantly advance our understanding of how CR impacts human aging at a molecular level.
• Further research using this resource is expected to uncover new insights into aging biology and potential interventions.