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Updated: Jun 14, 2025

Preparation of Enantiopure Non-Activated Aziridines and Synthesis of Biemamide B, D, and epiallo-Isomuscarine
Published on: June 13, 2022
Engineered Imine Reductase Catalyzed Enantiodivergent Synthesis of Alkylated Amphetamines
Yaqing Ma1,2,3, Shu-Shan Gao2,4, Xin Li2
1CAS Key Laboratroy of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Reseources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Abstract:
Less steric ketones exhibited low stereoselectivity toward M5 due to their difficulty in restricting the free rotation of the imine intermediate. An engineered enantio-complementary imine reductase from M5 was obtained with catalytic activity. We identified four key residues that play essential roles in controlling stereoselectivity. Two mutants, I149Y-W234L (up to 99%S ee) and L200M-F260M (up to 99%R ee), were achieved, showing excellent stereoselectivity toward the tested substrates, offering valuable biocatalysts for synthesizing alkylated amphetamines.
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