Periodic Acid Schiff Staining to Detect Mott Cells, Aberrant Plasma Cells Containing Immunoglobulin Inclusions Called

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Pedram Mahmoudi Aliabadi1, Khlowd Al-Qaisi1, Vishnu Reddy2

  • 1Deutsches Rheuma-Forschungszentrum Berlin, Berlin, Germany.

Current Protocols
|September 4, 2024
PubMed

Insights

This study presents a protocol using periodic acid Schiff (PAS) staining to detect Mott cells, which are plasma cells with immunoglobulin inclusions. This method is effective across various sample types for histopathological analysis.

Area of Science:

  • Histopathology
  • Immunology
  • Cell Biology

Background:

  • Hematoxylin and eosin (H&E) staining is standard for routine histopathology.
  • Periodic acid Schiff (PAS) staining detects polysaccharides and is crucial in immunology for evaluating kidney lesions.
  • PAS staining does not stain erythrocytes, aiding immune cell identification in the spleen.

Purpose of the Study:

  • To describe a protocol for detecting Mott cells, characterized by immunoglobulin inclusion bodies (Russell bodies).
  • To provide a reliable method for identifying these specific plasma cell alterations.

Main Methods:

  • Utilizes periodic acid Schiff (PAS) staining.
  • Applicable to formalin-fixed, paraffin-embedded tissue sections.
  • Also effective for frozen tissue sections, touch preparations, blood films, and cytocentrifuged cell smears.

Main Results:

  • Successful detection of Mott cells using the described PAS staining protocol.
  • Demonstrates the versatility of the protocol across different sample preparations.

Conclusions:

  • The PAS staining protocol provides an effective means for identifying Mott cells.
  • This method is adaptable for various histopathological and cytological sample types.
Keywords:
Mott cellRussell bodyimmunoglobulin inclusion bodyperiodic acid Schiff stainingplasma cell

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