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A gel casting technique allowing rapid and sensitive quantitation of antibodies by diffusion-in-gel enzyme-linked
Journal of Immunological Methods
|May 10, 1985
Abstract:
A technique is described which allows rapid and sensitive quantitation of antibodies by diffusion towards an antigen-coated surface through a gel of wedge-shaped profile. A sensitivity of 0.16 micrograms antibody per ml solution was obtained after 45 min incubation with stirring at 22 degrees C and 0.08 micrograms/ml could be detected after 90 min incubation with stirring or with incubation at 37 degrees C.