Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ultrahigh-Throughput Enrichment of Circulating Tumor Cells from Whole Blood.

ACS sensors·2026

Same author

Autonomous microfluidic labs: progress and prospects.

Lab on a chip·2026

Same author

Mastering Mechanical Measurements.

ACS sensors·2025

Same author

Measuring concentration and diffusivity within biomolecular condensates using calibration-free scanning fluorescence correlation spectroscopy.

Chemical science·2025

Same author

Mechanobiological regulation of T cells <i>via</i> transient viscoelastic microfluidic confinement.

Lab on a chip·2025

Same author

AI-assisted design of ligands for lipocalin-2.

Frontiers in immunology·2025

Same journal

Structural Hairpin Anchoring-Mediated TtAgo Activity Regulation for Programmable Biosensing.

Analytical chemistry·2026

Same journal

Digital Revitalization of a Legacy Linear Ion Trap System.

Analytical chemistry·2026

Same journal

An Interface-Regulated Electrochemical Biosensing Platform Based on the Cascade Amplification of Primer Exchange Reaction and CRISPR/Cas12a for Noninvasive Bladder Cancer Diagnosis.

Analytical chemistry·2026

Same journal

Spatially Resolved Diffusion NMR for Structurally Heterogeneous Materials.

Analytical chemistry·2026

Same journal

Direct Whole-Blood Multiplexing of Small Molecules via a Micelle-Enhanced Chemiluminescent Paper Sensor with Mesoporous Silica Membrane.

Analytical chemistry·2026

Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 14, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Robust Double Emulsions for Multicolor Fluorescence-Activated Cell Sorting.

Yun Ding¹, Giada Zoppi², Gaia Antonini²

¹Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093 Zürich, Switzerland.

Analytical Chemistry

|September 4, 2024

Summary

This summary is machine-generated.

This study introduces a simple workflow for high-throughput cell sorting using commercial fluorescence-activated cell sorters (FACS) and double emulsions. This method enables efficient screening of multicellular interactions for immune cell activation.

More Related Videos

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Related Experiment Videos

Last Updated: Jun 14, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Area of Science:

Biotechnology
Cell Biology
Immunology

Background:

Multicellular organisms rely on cell-cell interactions, such as T-cell receptor (TCR)-antigen interactions between T cells and antigen-presenting cells (APCs).
Fluorescence-activated droplet sorting (FADS) is a high-throughput method for screening cellular interactions, but current instruments have limitations in throughput and operational complexity.
Commercial fluorescence-activated cell sorters (FACS) offer high speed, sensitivity, and multiplexing but are underutilized for droplet sorting applications.

Purpose of the Study:

To develop a universally applicable and easy-to-implement workflow for generating double emulsions and performing multicolor cell sorting using a commercial FACS instrument.
To overcome the limitations of existing FADS technologies by leveraging the capabilities of commercial FACS machines.

Main Methods:

Development of a novel workflow for generating double emulsions compatible with commercial FACS instruments.
Implementation of multicolor cell sorting within the generated double emulsions.
Validation of the workflow's efficiency and applicability for screening cellular interactions.

Main Results:

Achieved a double emulsion detection rate exceeding 90%.
Enabled multicellular encapsulation and high-throughput immune cell activation sorting.
Demonstrated a simple and cost-effective method for advanced microfluidic applications.

Conclusions:

The presented droplet sorting strategy provides a universally applicable and simple-to-implement workflow for cell biology laboratories.
This approach enhances analytical throughput and simplifies operations compared to existing FADS techniques.
The method offers access to an advanced microfluidic toolbox with minimal effort and cost, facilitating research in cell-cell interactions and immune responses.