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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 14, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

High-throughput fluorescence lifetime imaging flow cytometry.

Hiroshi Kanno^1,2, Kotaro Hiramatsu^3,4, Hideharu Mikami^3,5

¹Department of Chemistry, The University of Tokyo, Tokyo, Japan. hkanno@g.ecc.u-tokyo.ac.jp.

Nature Communications

|September 4, 2024

Summary

This summary is machine-generated.

High-throughput fluorescence lifetime imaging microscopy (FLIM) flow cytometry now exceeds 10,000 cells per second. This breakthrough overcomes speed limitations, enhancing cellular analysis for biomedical research.

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Last Updated: Jun 14, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Area of Science:

Biomedical research
Laboratory medicine
Cellular analysis

Background:

Flow cytometry is crucial but limited by fluorescence intensity fluctuations.
Fluorescence lifetime imaging microscopy (FLIM) offers stable measurements but faces speed challenges for flow cytometry integration.

Purpose of the Study:

To overcome speed limitations in FLIM for high-throughput flow cytometry.
To enable advanced cellular analysis beyond traditional flow cytometry.

Main Methods:

Developed a high-throughput FLIM flow cytometry system exceeding 10,000 cells/sec.
Utilized dual intensity-modulated continuous-wave beam arrays with complementary modulation frequencies for fluorophore excitation.
Acquired fluorescence lifetime images of rapidly flowing cells.

Main Results:

Achieved over 10,000 cells per second for FLIM flow cytometry.
Successfully distinguished subpopulations in male rat glioma.
Captured dynamic changes in the cell nucleus induced by an anti-cancer drug.

Conclusions:

High-throughput FLIM flow cytometry significantly enhances cellular analysis capabilities.
Provides detailed insights into cellular functions, interactions, and environments.
Overcomes previous speed limitations for broader application in research and medicine.