Nymphaea alba leaf powder effectiveness in removing nisin from fermentation broth using docking and experimental...

The accumulation of nisin in the fermentation medium can reduce the process's productivity. This research studied the potential of Nymphaea alba leaf powder (NALP) as a hydrophobic biosorbent for efficient in-situ nisin adsorption from the fermentation medium by docking and experimental analysis. Molecular docking analysis showed that di-galloyl ellagic acid, a phytochemical compound found in N. alba, had the highest affinity towards nisin. Enhancements in nisin adsorption were seen following pre-treatment of NAPL with HCl and MgCl₂. A logistic growth model was employed to evaluate the growth dynamics of the biosorption capacity, offering valuable insights for process scalability. Furthermore, optimization through Response Surface Methodology elucidated optimal nisin desorption conditions by Liebig's law of the minimum, which posits that the scarcest resource governs production efficiency. Fourier Transform Infrared (FTIR) spectroscopy pinpointed vital functional groups involved in biosorption. Scanning electron microscopy revealed the changing physical characteristics of the biosorbent after exposure to nisin. The findings designate NALP as a feasible adsorbent for nisin removal from the fermentation broth, thus facilitating its application in the purification of other biotechnological products based on growth and production optimization principles.