Nymphaea alba leaf powder effectiveness in removing nisin from fermentation broth using docking and experimental...

Area of Science:

• Biotechnology
• Biochemistry
• Materials Science

Background:

• Nisin accumulation in fermentation media limits productivity.
• Developing efficient nisin removal methods is crucial for biotechnological processes.

Purpose of the Study:

• To investigate Nymphaea alba leaf powder (NALP) as a biosorbent for in-situ nisin adsorption.
• To analyze the phytochemical interactions and optimize biosorption/desorption conditions.

Main Methods:

• Molecular docking simulations to identify binding affinities.
• Experimental analysis including biosorbent pre-treatment (HCl, MgCl2).
• Logistic growth modeling for biosorption dynamics, Response Surface Methodology for optimization, FTIR, and SEM for characterization.

Main Results:

• Di-galloyl ellagic acid in NALP showed high affinity for nisin.
• Pre-treatment enhanced nisin adsorption capacity.
• Optimal desorption conditions were determined using Response Surface Methodology.
• FTIR and SEM confirmed functional groups and physical changes during biosorption.

Conclusions:

• Nymphaea alba leaf powder is a viable adsorbent for nisin removal from fermentation broth.
• NALP facilitates purification of biotechnological products by optimizing nisin removal.
• The study provides insights for scalable biosorption processes and product purification.