Related Experiment Video
Updated: Jun 14, 2025
Intratracheal Administration of Dry Powder Formulation in Mice
Published on: July 25, 2020
Treprostinil palmitil inhalation powder leverages endogenous lung enzymes to provide sustained treprostinil
Tam Nguyen1, Christina Chang1, David Cipolla1
1Department of Research, Insmed Incorporated, Bridgewater, NJ, USA.
Background:
To determine key enzymes enabling treprostinil palmitil (TP) conversion to treprostinil and the main converting sites in the respiratory system.
Research Design And Methods:
We performed in vitro activity assays to identify lung enzymes hydrolyzing TP, and cell-based assays and immunostainings to establish the likely locations within the lung.
Results:
Lipoprotein lipase (LPL) had greater activity than the other tested lung enzymes. Excess LPL activity was present both in vitro and at the target TP dose in vivo.
Conclusions:
LPL is likely the key enzyme enabling TP conversion. The rate-limiting step is likely the accessibility of TP and not the enzyme activity.
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