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Updated: Apr 6, 2026

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
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Engineered Cell Elongation Promotes Extracellular Electron Transfer of Shewanella Oneidensis.

Feng Li1, Huan Yu1, Baocai Zhang1

  • 1Frontier Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering, and School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 5, 2024
PubMed
Summary

Engineering electroactive microorganisms (EAMs) for cell elongation significantly boosts extracellular electron transfer (EET) and power output. This approach enhances microbial fuel cell performance and pollutant degradation capabilities.

Keywords:
biofilm formationcellular lengthc‐type cytochromesdivisomeextracellular electron transfer (EET)

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Area of Science:

  • Microbiology
  • Bioelectrochemistry
  • Synthetic Biology

Background:

  • Electroactive microorganisms (EAMs) are crucial for bioelectrochemical systems.
  • Extracellular electron transfer (EET) efficiency in EAMs limits their application.
  • Cellular morphology, specifically length, is a potential factor influencing EET.

Purpose of the Study:

  • To investigate the impact of programmed cell elongation on the EET of Shewanella oneidensis MR-1.
  • To engineer EAMs for enhanced power density and pollutant treatment capabilities.
  • To elucidate the molecular mechanisms underlying cell elongation-mediated EET enhancement.

Main Methods:

  • Genetic engineering of Shewanella oneidensis MR-1 to inhibit cell division using anti-sense RNAs or division inhibitors.
  • Electrophysiological measurements to quantify output current and power density.
  • Transcriptomic analysis to understand cellular changes.
  • Quorum sensing-based dynamic regulation for sustained growth and elongation.

Main Results:

  • Engineered strains exhibited significantly increased cellular length and output power density.
  • Cell elongation enhanced EET by upregulating NADH oxidation, the inner-membrane quinone pool, and c-type cytochromes.
  • Elongated cells showed increased hydrophobicity, facilitating biofilm formation.
  • Quorum sensing regulation enabled the generation of ultra-elongated cells (143.6 µm) with a 3.41-fold increase in power density (248.0 mW m⁻²).

Conclusions:

  • Programmed cell elongation is an effective strategy to enhance EET in EAMs.
  • This approach offers a novel avenue for improving microbial fuel cell performance and bioremediation.
  • The combination of cell elongation and quorum sensing provides a robust method for optimizing EAM function.