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Related Experiment Videos

Structure and sequence divergence of two archaebacterial genes.

D Cue, G S Beckler, J N Reeve

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1985
    PubMed
    Summary

    DNA sequence analysis of Methanococcus voltae and Methanococcus vannielii hisA genes reveals significant homology but suggests lower taxonomic relatedness compared to enteric bacteria.

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    Chromosome packaging by archaeal histones.

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    Area of Science:

    • Microbiology
    • Genomics
    • Archaea

    Background:

    • Methanogenic archaea are crucial in anaerobic environments.
    • Understanding gene organization and homology aids in taxonomic classification.
    • The hisA gene is essential for histidine biosynthesis.

    Purpose of the Study:

    • To compare the DNA sequences of the hisA gene region in Methanococcus voltae and Methanococcus vannielii.
    • To investigate genome organization and sequence homology between these two archaeal species.
    • To infer taxonomic relationships based on genetic similarity.

    Main Methods:

    • DNA sequencing of the hisA gene region.
    • Comparative sequence analysis to identify homologous regions and differences.
    • Analysis of genome organization, including open reading frames (ORFs) and intergenic regions.

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  • Codon usage bias analysis in A + T-rich genomes.

    • Main Results:

    • Similar genome organization with three ORFs and high A + T content in both species.
    • Significant DNA sequence homology observed for ORFHisA.
    • High preference for A and U at the third codon position.
    • Limited sequence homology in upstream regions, with distinct repetitive elements in M. voltae and M. vannielii.
    • Prokaryotic-like promoter sequences and a conserved ribosome binding site (GGTG) upstream of hisA genes.

    Conclusions:

    • Methanococcus voltae and Methanococcus vannielii exhibit conserved gene organization and significant hisA homology.
    • The observed differences in intergenic regions suggest distinct evolutionary paths.
    • Based on hisA homology, M. voltae and M. vannielii are taxonomically less related than enteric eubacteria.