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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: Jun 14, 2025

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
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Efficient RNA interference method by feeding in Brachionus plicatilis (Rotifera).

Yu Zhang1, Dongqi Kan1, Yang Zhou1

  • 1School of Marine Science and Engineering, Nanjing Normal University, No. 2 Xuelin Rd, Nanjing, 210023, People's Republic of China.

Biotechnology Letters
|September 5, 2024
PubMed
Summary

We developed an efficient RNA interference (RNAi) method for rotifers (Brachionus plicatilis) by feeding them dsRNA. This technique successfully reduced gene expression and affected survival, advancing rotifer research.

Keywords:
FeedingKnockdownKu70 & Ku80RNA interferenceRotifer

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Area of Science:

  • * Molecular biology
  • * Aquatic toxicology
  • * Geroscience

Background:

  • * Rotifers are valuable model organisms for ecological and toxicological studies.
  • * Limited gene manipulation tools hinder rotifer research.
  • * Efficient RNA interference (RNAi) is needed for functional gene studies.

Purpose of the Study:

  • * To establish a feeding-based RNAi method in the rotifer Brachionus plicatilis.
  • * To validate the method by targeting DNA repair genes (Bp-Ku70 & Ku80).

Main Methods:

  • * Utilized L4440 plasmid and RNase-deficient E. coli HT115 to produce dsRNA.
  • * Administered dsRNA to B. plicatilis via feeding.
  • * Assessed gene knockdown effects on DNA repair-related genes after UV exposure.

Main Results:

  • * Successfully achieved significant gene knockdown of Bp-Ku70 & Ku80 via feeding-based RNAi.
  • * Observed significant reductions in mRNA expression, fecundity, and survival rates post-RNAi.
  • * Demonstrated the efficacy of RNAi in response to DNA damage (UV radiation).

Conclusions:

  • * Feeding-based RNAi is a simple and effective method for gene knockdown in B. plicatilis.
  • * This advancement facilitates the use of rotifers as model organisms in biological research.
  • * The study provides a crucial tool for studying gene function in rotifers.