Two "turn on" fluorescence probes based on nitroso recognition group for detecting hydrogen sulfide

Area of Science:

• Analytical Chemistry
• Biochemistry
• Cell Biology

Background:

• Hydrogen sulfide (H₂S) is a crucial signaling molecule involved in various biological processes.
• Accurate detection of H₂S is essential for understanding its physiological and pathological roles.

Purpose of the Study:

• To design and synthesize novel "OFF-ON" fluorescence probes for sensitive and selective detection of H₂S.
• To evaluate the performance of the designed probes in terms of response rate, linearity, and detection limits.
• To demonstrate the application of the probes in biological systems for sensing endogenous and exogenous H₂S.

Main Methods:

• Design of "OFF-ON" fluorescence probes (YNO and TNO) utilizing a nitroso recognition group.
• Spectroscopic analysis to determine probe performance, including response rate, linearity, and detection limits.
• Application of the YNO probe for sensing H₂S in Hela cells, targeting the endoplasmic reticulum (ER).

Main Results:

• Both YNO and TNO probes exhibited outstanding response rates and a linear relationship between fluorescence intensity and H₂S concentration.
• YNO demonstrated a larger Stokes shift and longer emission wavelength compared to TNO.
• TNO achieved a lower limit of detection.
• YNO was successfully applied to detect endogenous and exogenous H₂S within Hela cells, specifically targeting the ER.

Conclusions:

• The developed YNO and TNO probes are effective tools for the sensitive detection of hydrogen sulfide.
• YNO offers advantages in terms of spectral properties and cellular imaging capabilities.
• These probes hold promise for further research into the biological functions of H₂S.