Specialized metabolism in St John's wort

The specialized metabolism of St. John's wort, Hypericum perforatum L., is a key focus in medicinal plant research due to its hallmark bioactive compounds hyperforin and hypericin. Known for its traditional medicinal uses dating back to ancient times, St. John's wort is currently used for mild depression therapy. Recent research works have shed light on the biosynthesis of various metabolites in this plant, such as flavonoids, xanthones, hyperforin, and hypericin. The elucidation of these pathways, along with the discovery of novel enzymes like hyperforin synthase, support the pharmaceutical research by enabling scalable production of bioactive compounds for the development of new drugs. Elucidation of the hyperforin biosynthesis based on single-cell RNA-seq is an approach that will be expanded and accelerate the gene discovery and full pathway reconstitution of plant specialized metabolites.