Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of DNA Repair02:25

Overview of DNA Repair

30.9K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
30.9K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.0K
Homologous Recombination02:31

Homologous Recombination

50.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.3K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.5K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.5K
Nucleosome Remodeling02:54

Nucleosome Remodeling

9.0K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.0K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sodium alginate for wound healing applications: A review.

Polimery w medycynie·2026
Same author

Salvage total hip arthroplasty following hip fracture treatment-A systematic review and meta-analysis of the role of fracture type and implant design.

Journal of clinical orthopaedics and trauma·2026
Same author

Pregnancy outcomes in RA and SLE patients: analysis of the 2019 Nationwide Inpatient Sample Database.

EULAR rheumatology open·2026
Same author

Ubiquitin-dependent degradation of p27Kip1 and p21Waf1/Cip1 by AMBRA1 ensures G1 and S phase progression and limits replication stress.

Nucleic acids research·2026
Same author

USP Gene Network Modulation and Osmoprotection Define Salt Resilience in Chenopodium quinoa Genotypes.

Scientific reports·2026
Same author

Machine learning-based modeling of pharmaceutical sorption in soils: Integrating conformal prediction and Shapley additive explanations analysis for robust risk assessment.

Environmental toxicology and chemistry·2026

Related Experiment Video

Updated: Jun 14, 2025

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

9.2K

Exploring the structural landscape of DNA maintenance proteins.

Kenneth Bødkter Schou1,2,3, Samuel Mandacaru4, Muhammad Tahir4

  • 1Genome Integrity, Danish Cancer Institute, Danish Cancer Society, Strandboulevarden 49, 2100, Copenhagen, Denmark. kensch@cancer.dk.

Nature Communications
|September 5, 2024
PubMed
Summary

This study systematically surveys genome maintenance (GM) proteins using profile-to-profile models, revealing novel candidates and domains. The findings suggest that the full repertoire of genome stability caretakers remains underestimated.

More Related Videos

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.2K
Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

2.7K

Related Experiment Videos

Last Updated: Jun 14, 2025

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

9.2K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.2K
Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

2.7K

Area of Science:

  • Genomics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Genome maintenance (GM) proteins are crucial for cellular stability.
  • Conventional evolutionary annotation relies on sequence similarity, often failing for distantly related proteins.

Purpose of the Study:

  • To systematically apply sensitive profile-to-profile methods for evolutionary annotation of GM proteins.
  • To identify novel GM protein candidates and annotate protein domains across species.

Main Methods:

  • Utilized iterative profile-to-profile methods for homology detection.
  • Systematically surveyed GM proteins from bacteria to humans.
  • Performed experimental validation of identified domains in specific protein families.

Main Results:

  • Identified multiple novel GM protein candidates.
  • Annotated domains in established GM proteins, including PARP, OB-fold, Macro, TUDOR, SAP, BRCT, KU, MYB (SANT), and nuclease domains.
  • Experimentally validated OB-fold and MIS18 (Yippee) domains in SPIDR and FAM72 families, respectively.

Conclusions:

  • The repertoire of genome stability caretakers is larger and less understood than previously thought.
  • Profile-to-profile methods are effective for uncovering evolutionary relationships of GM proteins.
  • Further research is needed to fully appreciate the diversity and function of GM proteins.