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Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
Published on: November 21, 2015
Biological function and potential application of PANoptosis-related genes in colorectal carcinogenesis
Xuan Yu1,2, Yongfu Shao1,2, Haotian Dong2
1Department of Gastroenterology, the First Affiliated Hospital of Ningbo University, Ningbo, 315020, China.
Abstract:
PANoptosis induces programmed cell death (PCD) through extensive crosstalk and is associated with development of cancer. However, the functional mechanisms, clinical significance, and potential applications of PANoptosis-related genes (PRGs) in colorectal cancer (CRC) have not been fully elucidated. Functional enrichment of key PRGs was analyzed based on databases, and relationships between key PRGs and the immune microenvironment, immune cell infiltration, chemotherapy drug sensitivity, tumor progression genes, single-cell cellular subgroups, signal transduction pathways, transcription factor regulation, and miRNA regulatory networks were systematically explored. This study identified 5 key PRGs associated with CRC: BCL10, CDKN2A, DAPK1, PYGM and TIMP1. Then, RT-PCR was used to verify expression of these genes in CRC cells and tissues. Clinical significance and prognostic value of key genes were further verified by multiple datasets. Analyses of the immune microenvironment, immune cell infiltration, chemotherapy drug sensitivity, tumor progression genes, single-cell cellular subgroups, and signal transduction pathways suggest a close relationship between these key genes and development of CRC. In addition, a novel prognostic nomogram model for CRC was successfully constructed by combining important clinical indicators and the key genes. In conclusion, our findings offer new insights for understanding the pathogenesis of CRC, predicting CRC prognosis, and identifying multiple therapeutic targets for future CRC therapy.
Insights
This study identifies five key PANoptosis-related genes (PRGs) linked to colorectal cancer (CRC) development and prognosis. These PRGs offer potential therapeutic targets and aid in predicting CRC patient outcomes.
Area of Science:
- Molecular Biology
- Oncology
- Immunology
Background:
- PANoptosis, a form of programmed cell death (PCD), is implicated in cancer development.
- The specific roles of PANoptosis-related genes (PRGs) in colorectal cancer (CRC) remain underexplored.
- Understanding PRGs is crucial for advancing CRC pathogenesis insights and therapeutic strategies.
Purpose of the Study:
- To elucidate the functional mechanisms and clinical significance of PRGs in CRC.
- To identify key PRGs associated with CRC development, progression, and patient prognosis.
- To explore the relationships between PRGs and the tumor immune microenvironment, drug sensitivity, and signaling pathways.
Main Methods:
- Bioinformatic analysis of PRGs and functional enrichment.
- Exploration of PRG correlations with immune microenvironment, cell infiltration, and drug sensitivity.
- RT-PCR validation of key PRG expression in CRC tissues and cells.
- Construction of a prognostic nomogram model for CRC.
Main Results:
- Five key PRGs (BCL10, CDKN2A, DAPK1, PYGM, TIMP1) were identified as significantly associated with CRC.
- These PRGs demonstrated strong correlations with immune cell infiltration, tumor progression, and chemotherapy sensitivity.
- A novel prognostic nomogram integrating clinical indicators and key PRGs was developed for CRC.
Conclusions:
- The identified key PRGs are integral to CRC pathogenesis and progression.
- These PRGs serve as potential biomarkers for predicting CRC prognosis.
- The findings highlight PRGs as promising therapeutic targets for colorectal cancer treatment.
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