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Related Experiment Video

Updated: Jun 14, 2025

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

YIPF2 regulates genome integrity.

Xiao Zhang^1,2,3,4, Tao Wang^5,6,7

¹Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint, Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

Cell & Bioscience

|September 5, 2024

Summary

The Golgi protein YIPF2 is crucial for maintaining genome stability by supporting DNA repair. Its presence protects cells from DNA damage and promotes recovery, offering insights into aging and disease interventions.

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Last Updated: Jun 14, 2025

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Published on: January 14, 2016

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Published on: February 3, 2022

Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

Genome integrity is vital for preventing aging and disease.
Understanding DNA repair mechanisms is key to developing interventions.
Novel factors in genome stability maintenance require identification.

Purpose of the Study:

To identify novel factors involved in maintaining genome stability using a whole-genome RNAi screen.
To investigate the role of the Golgi protein YIPF2 in genome integrity.
To explore the therapeutic potential of YIPF2 in DNA damage response and aging.

Main Methods:

Whole-genome RNAi screening to identify potential target genes.
Analysis of gene functions related to cell cycle, proteasome, and spliceosomes.
Investigating the impact of YIPF2 depletion and overexpression on DNA repair and cellular senescence.

Main Results:

A whole-genome RNAi screen identified novel factors involved in genome stability.
The Golgi protein YIPF2 was found to be critical for maintaining genome stability.
YIPF2 depletion impaired homologous recombination (HR) repair, triggering DNA damage responses and cellular senescence.
YIPF2 overexpression enhanced cellular recovery from DNA damage.

Conclusions:

The Golgi protein YIPF2 plays a critical role in maintaining genome stability.
YIPF2 is essential for effective homologous recombination (HR) repair.
An intact Golgi apparatus with YIPF2 provides a protective effect on genome integrity, with implications for aging and disease.
YIPF2 represents a potential therapeutic target for enhancing DNA repair and combating age-related decline.