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  11. Analysis Of Mirnas Involved In Mouse Brain Injury Upon Coxsackievirus A6 Infection.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Analysis Of Mirnas Involved In Mouse Brain Injury Upon Coxsackievirus A6 Infection.

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Analysis of miRNAs involved in mouse brain injury upon Coxsackievirus A6 infection.

Yihao Sun1,2, Yilin Hao1, Jie Wu2

  • 1Department of Biopharmacy, College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China.

Frontiers in Cellular and Infection Microbiology
|September 6, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Coxsackievirus A6 (CV-A6) causes severe hand, foot, and mouth disease (HFMD) and central nervous system (CNS) injury. This study reveals dysregulated microRNAs (miRNAs) and pathways involved in CV-A6-induced brain damage in mice.

Keywords:
Coxsackievirus A6 (CV-A6)braincentral nervous systemhand foot and mouth disease (HFMD)

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Area of Science:

  • Neuroscience
  • Virology
  • Molecular Biology

Background:

  • Coxsackievirus A6 (CV-A6) is a leading cause of hand, foot, and mouth disease (HFMD), associated with severe neurological complications like encephalitis and meningitis.
  • Previous studies show CV-A6 infection in neonatal mice leads to paralysis and death, but the mechanisms of nervous system injury are unclear.
  • MicroRNAs (miRNAs) are known to play critical roles in various viral infections and host responses.

Purpose of the Study:

  • To investigate the role of microRNAs (miRNAs) in the pathogenesis of central nervous system (CNS) injury induced by Coxsackievirus A6 (CV-A6).
  • To identify differentially expressed miRNAs and their target genes in the brain following CV-A6 infection.
  • To elucidate the molecular pathways involved in CV-A6-induced neuroinflammation and neuronal damage.
miRNA

Main Methods:

  • Comparative miRNA profiling of brain tissues from CV-A6 infected and control mice using qRT-PCR.
  • Prediction of miRNA target genes and pathway analysis using GO and KEGG enrichment.
  • Experimental validation of miRNA-mRNA interactions via double luciferase assays and Western Blotting.

Main Results:

  • Identification of 175, 198, and 78 differentially expressed miRNAs at 2, 4, and 2 vs 4 days post-infection, respectively.
  • GO and KEGG analyses revealed enrichment of pathways related to transcriptional regulation, neuronal necrosis, and immune responses.
  • Validation of miRNA-mRNA interactions and confirmation of functional enrichment of target genes.

Conclusions:

  • CV-A6-induced CNS injury involves complex mechanisms including transcriptional dysregulation, neuronal death, inflammation, and immune responses.
  • Multiple pathways, such as glial activation, synaptic destruction, and blood-brain barrier disruption, contribute to neurodegeneration.
  • The identified dysregulated miRNAs and signaling pathways offer crucial insights into CV-A6 pathogenesis and potential therapeutic targets.