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The mechanical properties measurement could be affected by forceps: a technical note on sampling human annulus
Tianchi Zhou1,2, Bowei Xiao1,2, Juying Huang3,2
1Department of Orthopaedic Surgery, Beijing Tiantan Hospital, Capital Medical University, No. 119 South 4th Ring West Road, Fengtai District, Beijing, 100070, China.
Summary
Forceps sampling significantly alters the micromechanical properties of annulus fibrosus extracellular matrix. Bistoury sampling is recommended for accurate biomechanical studies of cervical degenerative disease.
Area of Science:
- Biomaterials Science
- Biomechanics
- Spinal Research
Background:
- The annulus fibrosus (AF) is a critical component of the intervertebral disc, providing structural integrity.
- Degenerative changes in the AF contribute to various spinal pathologies.
- Accurate assessment of AF mechanical properties is essential for understanding disease mechanisms.
Purpose of the Study:
- To compare the micromechanical properties of human annulus fibrosus samples obtained via forceps versus bistoury.
- To determine if the sampling method influences the measured biomechanical characteristics of the AF extracellular matrix (ECM).
Main Methods:
- Human annulus fibrosus tissues were collected during surgery using either a bistoury or nucleus pulposus forceps.
- Atomic Force Microscopy (AFM) was employed to measure the elastic modulus and stiffness of the AF ECM.
- Tissues were histologically analyzed to assess structural integrity post-sampling.
Main Results:
- Histological examination revealed no significant differences between forceps and bistoury groups.
- Samples obtained by forceps exhibited significantly lower elastic modulus (0.41 ± 0.08 MPa) compared to bistoury samples (0.53 ± 0.13 MPa).
- Similarly, forceps-acquired samples showed significantly reduced stiffness (0.024 ± 0.003 N/m) versus bistoury samples (0.037 ± 0.003 N/m).
Conclusions:
- Forceps sampling significantly compromises the micromechanical properties of the annulus fibrosus ECM.
- The bistoury sampling method is recommended for reliable experimental investigation of AF micromechanics.
- These findings are crucial for accurate research into cervical degenerative disc disease.
