Related Experiment Video
Updated: Jun 14, 2025

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
Development of a cost-effective confocal Raman microscopy with high sensitivity
Ting Pan1, Tian-Yu Gao2, Xiang-Hua Fan2
1CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicines of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, 730000, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
We developed a cost-effective confocal Raman microscopy system that achieves high sensitivity, comparable to expensive scientific instruments. This breakthrough makes advanced material identification more accessible for broader applications.
Area of Science:
- Spectroscopy
- Materials Science
- Analytical Chemistry
Background:
- Confocal Raman microscopy is vital for material identification but hampered by weak signals.
- Existing instruments are either inexpensive with low sensitivity or highly sensitive but prohibitively costly.
- This cost-sensitivity trade-off limits the widespread adoption of Raman spectroscopy.
Purpose of the Study:
- To develop a cost-effective confocal Raman microscopy system with high sensitivity.
- To bridge the gap between affordable and high-performance Raman instrumentation.
- To enable broader applications of Raman spectroscopy in daily life and research.
Main Methods:
- Utilized a readily available laser diode for excitation.
- Employed a high-throughput, lens-grating-lens spectrometer for spectral analysis.
- Incorporated a cooled linear charge-coupled device (CCD) detector for enhanced sensitivity.
Main Results:
- Achieved high-quality Raman spectra with good spectral resolution.
- Demonstrated a signal-to-noise ratio (SNR) > 10:1 for the Si (111) wafer peak, comparable to high-end systems.
- Validated universal application capability across organic molecules, minerals, and polymers.
Conclusions:
- The developed compact Raman instrument offers a cost-effective solution for sensitive material identification.
- The system provides performance rivaling expensive scientific-grade instruments.
- This innovation expands the accessibility and applicability of confocal Raman microscopy.
More Related Videos
Related Concept Videos
Confocal Fluorescence Microscopy
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Super-resolution Fluorescence Microscopy
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

