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LSD1 inhibits the invasion and migration of breast cancer through exosomes
Nan Zhang1, Zhongyu Chen1, Benkai Xin1
1China-Japan Union Hospital of Jilin University, Jilin University, Changchun, 130033, Jilin, China.
Abstract:
Metastasis accounts for almost 90% of breast cancer-related fatalities, making it frequent malignancy and the main reason of tumor mortality globally among women. LSD1 is a histone demethylase, which plays an important role in breast cancer. In order to explore the effect of LSD1 on invasion and migration of breast cancer, we treated breast cancer cells with MCF7 and T47D exosomes knocked down by LSD1, and the invasion and migration of breast cancer cells were significantly enhanced. This phenomenon indicates that LSD1 can inhibit the invasion and migration of breast cancer cells. miR-1290 expression was downregulated in LSD1 knockdown MCF7 exosomes. By analyzing the database of miR-1290 target gene NAT1, we verified that miR-1290 could regulate the expression of NAT1. These data provide fresh insights into the biology of breast cancer therapy by demonstrating how the epigenetic factor LSD1 stimulates the breast cancer cells' invasion and migration via controlling exosomal miRNA.
Insights
Lysine-specific demethylase 1 (LSD1) normally inhibits breast cancer cell invasion and migration. Knocking down LSD1 in exosomes enhances these processes by downregulating miR-1290, impacting breast cancer progression.
Area of Science:
- Oncology
- Epigenetics
- Molecular Biology
Background:
- Metastasis is a major cause of breast cancer mortality.
- Lysine-specific demethylase 1 (LSD1) is an epigenetic factor implicated in various cancers.
- The role of LSD1 in breast cancer cell invasion and migration requires further elucidation.
Purpose of the Study:
- To investigate the effect of LSD1 on breast cancer cell invasion and migration.
- To explore the underlying molecular mechanisms involving exosomal microRNAs.
Main Methods:
- Utilized MCF7 and T47D breast cancer cell lines.
- Performed knockdown of LSD1 in exosomes.
- Analyzed exosomal microRNA expression and target gene regulation.
- Assessed cell invasion and migration assays.
Main Results:
- LSD1 knockdown in exosomes significantly enhanced breast cancer cell invasion and migration.
- Downregulation of miR-1290 was observed in LSD1-knockdown MCF7 exosomes.
- miR-1290 was confirmed to regulate the expression of its target gene, NAT1.
Conclusions:
- LSD1 acts as an inhibitor of breast cancer cell invasion and migration.
- LSD1 influences breast cancer progression through the regulation of exosomal miR-1290.
- This study offers insights into epigenetic regulation in breast cancer metastasis and potential therapeutic targets.
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