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Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...

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Related Experiment Video

Updated: Jun 14, 2025

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

The replication crisis has led to positive structural, procedural, and community changes.

Max Korbmacher^1,2,3, Flavio Azevedo^4,5, Charlotte R Pennington⁶

¹Department of Health and Functioning, Western Norway University of Applied Sciences, Bergen, Norway.

Communications Psychology

|September 6, 2024

Summary

The behavioral, cognitive, and social sciences faced a replication crisis due to low success rates in large studies. This perspective reframes the crisis as a credibility revolution, highlighting positive changes and a path forward for research integrity.

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Last Updated: Jun 14, 2025

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Area of Science:

Behavioral Science
Cognitive Science
Social Science

Background:

Large-scale replication projects revealed lower-than-expected success rates.
This led to a 'replication crisis' in behavioral, cognitive, and social sciences.

Purpose of the Study:

Reframe the 'replication crisis' as a 'credibility revolution'.
Focus on positive structural, procedural, and community-driven changes.
Outline a path for ongoing advancements in research integrity.

Main Methods:

Perspective piece analyzing the implications of replication failures.
Review of emerging positive changes within scientific communities.
Conceptual framework for understanding the 'credibility revolution'.

Main Results:

The replication crisis is reframed as a catalyst for positive change.
Significant structural, procedural, and community-driven improvements are identified.
The 'credibility revolution' is fostering a more robust research environment.

Conclusions:

The challenges posed by replication failures have spurred a necessary evolution in scientific practices.
These changes promise a long-term positive impact on the reliability and trustworthiness of research.
Continued focus on transparency and rigorous methodology is crucial.