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Related Experiment Videos

Deficient alternative complement pathway activity in newborn sera.

E L Mills, B Björksteń, P G Quie

    Pediatric Research
    |December 1, 1979
    PubMed
    Summary
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    Neonatal bacterial sepsis susceptibility may stem from underdeveloped alternative complement pathway function. This pathway is crucial for opsonizing certain bacteria, unlike the classical pathway, which appears adequate in newborns.

    Area of Science:

    • Immunology
    • Neonatal Medicine
    • Microbiology

    Background:

    • Neonates exhibit increased susceptibility to bacterial infections, often linked to diminished serum opsonic activity.
    • Limited research compares the functional capacity of classical versus alternative complement pathways in neonatal serum.

    Purpose of the Study:

    • To investigate and compare the functional activity of the classical and alternative complement pathways in neonatal serum.
    • To determine the role of complement pathway deficiencies in neonatal susceptibility to bacterial infections, specifically E. coli.

    Main Methods:

    • Assessed serum opsonic activity by measuring the uptake of radiolabeled Escherichia coli.
    • Utilized E. coli strains requiring either the classical or alternative complement pathway for opsonization.

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  • Analyzed nine maternal-infant serum pairs, including hemagglutination inhibition antibody titers.

    • Main Results:

    • Alternative complement pathway activity was significantly lower in neonatal sera (16.8% E. coli uptake) compared to maternal (54%) and control sera (45%).
    • Classical complement pathway activity was comparable between maternal and neonatal sera for E. coli strains requiring this pathway.
    • No correlation was found between hemagglutination inhibition antibody titers and phagocytosis levels.

    Conclusions:

    • Neonatal serum possesses adequate classical complement pathway components for E. coli opsonization.
    • Reduced alternative complement pathway activity in neonates may significantly contribute to their vulnerability to bacterial sepsis.