First hip hemiarthroplasty in a Göttingen Minipig; surgical and post-mortem protocol
Katrine Top Hartmann1, Anders Odgaard2,3, Ulrik Kragegaard Knudsen2
1Department of Veterinary- and Animal Sciences, University of Copenhagen, Grønnegårdsvej 7, 1870, Frederiksberg C, Denmark. katoh@sund.ku.dk.
Background:
Prosthetic joint infections (PJI) are recalcitrant, hard-to-treat infections and severe complications of joint arthroplasty. Therefore, there is a need to develop new effective treatment strategies, and animal models of high clinical relevance are needed. This study aimed to develop a detailed surgical protocol for hip hemiarthroplasty in Göttingen minipigs and a thorough post-mortem sampling protocol to pave the way for creating a minipig PJI model.
Methods:
Three adult female Göttingen minipigs underwent surgery with insertion of a hip hemiarthroplasty, using the anterior approach to the hip joint. After surgery the minipigs were followed closely with daily clinical evaluation and gait scoring. Comprehensive post-mortem analyses were performed with evaluation of macroscopic lesions, microbiology, synovial fluid analysis and histology.
Results:
The study resulted in the first Göttingen minipig with hip hemiarthroplasty and identified several points of awareness when inserting a hip prosthesis in minipigs, especially the high risk of joint dislocation. A spontaneous PJI occurred in one of the minipigs, revealing an impaired ability of the immune cells to reach the bacteria at the bone-prosthesis interface.
Conclusion:
The present study provides a detailed description of surgical technique and post-mortem sampling and validates the suitability of the hip hemiarthroplasty minipig model for future experimental modeling of PJI.
Insights
Researchers developed a hip hemiarthroplasty protocol in Göttingen minipigs, creating a valuable model for studying prosthetic joint infections (PJI) and immune responses at the bone-prosthesis interface.
Area of Science:
- Biomedical Engineering
- Comparative Pathology
- Surgical Innovation
Background:
- Prosthetic joint infections (PJI) are challenging complications of joint arthroplasty requiring novel treatment strategies.
- Developing clinically relevant animal models is crucial for advancing PJI research.
- Existing models may not fully replicate the complexities of human PJI.
Purpose of the Study:
- To establish a detailed surgical protocol for hip hemiarthroplasty in Göttingen minipigs.
- To develop a comprehensive post-mortem sampling protocol for PJI research.
- To validate the Göttingen minipig as a model for experimental PJI.
Main Methods:
- Hip hemiarthroplasty was performed in three Göttingen minipigs using an anterior surgical approach.
- Post-operative monitoring included daily clinical evaluations and gait scoring.
- Extensive post-mortem analyses encompassed macroscopic examination, microbiology, synovial fluid analysis, and histology.
Main Results:
- The study successfully established hip hemiarthroplasty in Göttingen minipigs, noting a high risk of joint dislocation.
- A spontaneous PJI case highlighted impaired immune cell migration to the bone-prosthesis interface.
- The model provided insights into the challenges of treating PJI.
Conclusions:
- A detailed surgical and post-mortem sampling protocol for hip hemiarthroplasty in minipigs was successfully developed.
- The Göttingen minipig model is validated as suitable for future experimental modeling of prosthetic joint infections.
- This model offers a platform for investigating PJI pathogenesis and testing new therapies.
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