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  1. Home
  3. Genome-wide Identification Of Cannabinoid Biosynthesis Genes In Non-drug Type Cannabis (cannabis Sativa L.) Cultivar.
  1. Home
  3. Genome-wide Identification Of Cannabinoid Biosynthesis Genes In Non-drug Type Cannabis (cannabis Sativa L.) Cultivar.

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Genome-wide identification of cannabinoid biosynthesis genes in non-drug type Cannabis (Cannabis sativa L.) cultivar.

Benny Jian Rong Sng1,2, Yu Jeong Jeong3, Sing Hui Leong1

  • 1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore.

Journal of Cannabis Research
|September 7, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Hemp Cheungsam flowers show high cannabidiolic acid (CBDA) synthase expression, leading to low Δ9-tetrahydrocannabinolic acid (THCA) content. This study reveals the cannabinoid biosynthesis pathway in hemp, identifying key gene orthologs.

Keywords:
Cannabis sativaHempRNA-seq

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Area of Science:

  • Plant Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Cannabis sativa is classified as hemp or marijuana based on Δ9-tetrahydrocannabinolic acid (THCA) levels.
  • Hemp Cheungsam, a low-THCA cultivar, lacks detailed investigation into its cannabinoid biosynthesis gene expression.
  • Understanding hemp's genetic pathways is crucial for distinguishing it from marijuana and for industrial applications.

Purpose of the Study:

  • To investigate the transcriptome and cannabinoid biosynthesis pathway gene expression in hemp Cheungsam.
  • To identify homologs of cannabinoid biosynthesis genes in hemp Cheungsam.
  • To elucidate the molecular basis for low THCA content in hemp Cheungsam.

Main Methods:

  • RNA-sequencing (RNA-seq) was performed on hemp Cheungsam flower, leaf, and stem tissues.
  • Expression levels of cannabinoid biosynthesis pathway genes were analyzed across different tissue types.
  • Multiple sequence alignment and conserved domain analyses were employed to identify gene homologs.

Main Results:

  • The cannabinoid biosynthesis pathway is predominantly expressed in hemp Cheungsam flowers.
  • Cannabidiolic acid (CBDA) synthase exhibited significantly higher expression than THCA synthase and cannabichromenic acid (CBCA) synthase.
  • Sequence analysis confirmed the presence of orthologs for key cannabinoid biosynthesis genes in hemp Cheungsam.

Conclusions:

  • Cannabinoid biosynthesis in hemp Cheungsam is primarily localized to floral tissues.
  • High CBDA synthase expression and low THCA/CBCA synthase expression correlate with hemp Cheungsam's low THCA content.
  • The identified gene orthologs provide a foundation for understanding cannabinoid production in hemp.