Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gastrulation01:56

Gastrulation

56.9K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
56.9K
Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bio-based sodium alginate/polyvinyl alcohol double-network aerogels reinforced by phytic acid and MXene for flame-retardant thermal insulation.

International journal of biological macromolecules·2026
Same author

Magnetic field-assisted freezing mitigates quality deterioration in oyster meat (Crassostrea hongkongensis) by inducing the formation of uniform ice crystals and preserving water-holding capacity.

Food chemistry·2026
Same author

Cross-domain transfer of trehalose biosynthesis genes contributes to adaptation in high-altitude environments.

National science review·2026
Same author

Effects of heat treatment on the characteristics of low-molecular-weight oyster peptides (LOPs) W<sub>1</sub>/O/W<sub>2</sub> double emulsions: Physicochemical, interfacial, and rheological properties.

Food chemistry·2026
Same author

Non-invasive detection of hTERT mRNA in deep-cough swabs for early diagnosis of lung cancer.

Frontiers in molecular biosciences·2026
Same author

The First Systematic Histological Observation of the Male Antarctic Krill (Euphausia Superba) Reproductive System.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Salt priming coordinates transcriptional and epigenetic states for enhanced salt tolerance in mung bean (Vigna radiata).

Communications biology·2026
Same journal

A male-derived volatile sex pheromone in Caenorhabditis nematodes identified through its mimicry by a predator.

Communications biology·2026
Same journal

Revalidation of Manis aurita based on integrative genomic and morphological evidence.

Communications biology·2026
Same journal

Presenilin-1 controls glycolysis and identity of pancreatic beta cells.

Communications biology·2026
Same journal

Base editing-derived models of human WDR34 and WDR60 disease alleles replicate retrograde intraflagellar transport (IFT) and hedgehog signaling defects.

Communications biology·2026
Same journal

Butterflies with low thermoregulatory capacity show greatest upwards range shifts along an elevational gradient.

Communications biology·2026
See all related articles

Related Experiment Video

Updated: Jun 14, 2025

Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
06:46

Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development

Published on: September 4, 2014

14.9K

PHD2 safeguards modest mesendoderm development.

Meng Li1, Huaizhang Jin1, Yun Zhao1

  • 1College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.

Communications Biology
|September 7, 2024
PubMed
Summary
This summary is machine-generated.

Prolyl hydroxylase domain 2 (PHD2) is crucial for mesendoderm specification by regulating hypoxia-inducible factor 1-alpha (HIF-1α). This study reveals PHD2

More Related Videos

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
06:21

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Published on: April 25, 2019

9.3K
An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
07:45

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

Published on: January 20, 2013

9.8K

Related Experiment Videos

Last Updated: Jun 14, 2025

Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
06:46

Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development

Published on: September 4, 2014

14.9K
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
06:21

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Published on: April 25, 2019

9.3K
An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
07:45

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

Published on: January 20, 2013

9.8K

Area of Science:

  • Developmental Biology
  • Cellular Biology
  • Molecular Biology

Background:

  • Hypoxia is a key feature of the uterus, and hypoxia-inducible factor 1-alpha (HIF-1α) opposes mesendoderm specification.
  • Prolyl hydroxylase domain 2 (PHD2) modulates HIF-1α levels in response to oxygen changes.
  • The interplay between hypoxia, HIF-1α, and mesendoderm specification suggests a potential role for PHD2.

Purpose of the Study:

  • To investigate the role of PHD2 in mesendoderm specification during early embryonic development.
  • To elucidate the regulatory mechanisms and pathways influenced by PHD2 in this process.

Main Methods:

  • Expression analysis of PHD2 in mouse embryos.
  • Functional studies using mouse embryonic stem cells (mESCs), embryoids, and mouse embryos.
  • Single-cell RNA sequencing to analyze cellular transitions.
  • Investigation of downstream signaling pathways, including Wnt/β-catenin and upstream microRNA regulation (miR-429).

Main Results:

  • PHD2 expression patterns mirrored hypoxia and were distinct from HIF-1α.
  • PHD2 was found to negatively regulate mesendoderm specification.
  • Single-cell RNA sequencing demonstrated PHD2's role in the epiblast to mesendoderm transition.
  • PHD2's downstream effects involved the HIF-1α-regulated Wnt/β-catenin pathway and were upstream regulated by miR-429.

Conclusions:

  • PHD2 plays an essential role in mesendoderm specification.
  • PHD2 interacts with hypoxia and HIF-1α signaling pathways during embryonic development.
  • PHD2's function is integrated with miR-429 and the Wnt/β-catenin pathway in regulating cell fate transitions.