Phytochemical-mediated modulation of cancer-associated proteins: an integrated in-silico and in-vitro study

Area of Science:

• Oncology
• Pharmacology
• Computational Biology

Background:

• Cancer is a major global health challenge with increasing incidence.
• Existing treatments like chemotherapy and radiation have limitations and side effects.
• There is a need for novel, multi-targeted therapeutic agents.

Purpose of the Study:

• To investigate the multi-target therapeutic potential of seven phytochemicals against critical cancer-associated proteins.
• To evaluate the efficacy of these phytochemicals using computational and in-vitro methods.
• To identify promising phytochemicals for further development as anticancer agents.

Main Methods:

• Computational docking studies were performed to analyze binding interactions between phytochemicals and target proteins.
• In-vitro experiments were conducted on cancer cell lines (A549) and normal cells (Vero).
• Cytotoxicity was assessed on Vero cells, and anticancer activity (proliferation inhibition, apoptosis induction) was evaluated on A549 cells.

Main Results:

• Phytochemicals exhibited concentration-dependent cytotoxicity on Vero cells.
• Specific phytochemicals demonstrated significant anticancer activity against A549 cells.
• These compounds were effective in inhibiting cancer cell proliferation and inducing apoptosis.

Conclusions:

• Certain phytochemicals possess multi-targeted therapeutic potential against cancer.
• The identified phytochemicals show promise as novel anticancer agents.
• Further research into these compounds could lead to improved cancer therapies.