Simultaneous detection of ovarian cancer related miRNA biomarkers with carboxylated graphene oxide modified electrochemical biosensor platform

Ezgi Kivrak ¹, Pinar Kara ²

⁰Faculty of Pharmacy, Department of Analytical Chemistry, Ege University, 35100 Izmir, Bornova, Turkey; Graduate School of Natural and Applied Sciences, Department of Biomedical Technologies, Ege University, 35100 Izmir, Bornova, Turkey.

Bioelectrochemistry (Amsterdam, Netherlands) +

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September 8, 2024

View abstract on PubMed

Summary

A novel electrochemical nanobiosensor detects two microRNAs (miRNAs) linked to ovarian cancer. This sensitive platform offers potential for earlier disease diagnosis and improved treatment strategies.

Area Of Science

Biomedical Engineering
Molecular Diagnostics
Nanotechnology

Background

Ovarian cancer, a high-mortality gynecological cancer, is often diagnosed late due to non-specific biomarkers like CA-125.
The miR-200 family of RNA molecules is upregulated in ovarian cancer, suggesting potential as specific biomarkers.
Multiplexed detection of multiple biomarkers is crucial for accurate diagnosis, overcoming limitations of single-marker approaches.

Purpose Of The Study

To develop a sensitive electrochemical nanobiosensor for simultaneous detection of miR-200c and miR-141.
To establish a point-of-care system for early ovarian cancer diagnosis.
To explore novel diagnostic tools beyond traditional immunoassay techniques.

Main Methods

Fabrication of an electrochemical nanobiosensor using carboxylated graphene oxide modified pencil graphite electrodes.
Immobilization of methylene blue and ferrocene labeled hairpin DNA probes for specific miRNA hybridization.
Utilizing a "signal-off" approach with Square Wave Voltammetry for quantitative detection.

Main Results

The nanobiosensor achieved multiplexed and simultaneous detection of miR-200c and miR-141.
Demonstrated a linear detection range from 0.1 pM to 10 nM for both miRNAs.
Achieved low limits of detection: 0.029 pM for miR-141 and 0.026 pM for miR-200c.

Conclusions

The developed electrochemical nanobiosensor platform shows promise for early ovarian cancer diagnosis.
This multi-biomarker detection approach could lead to more effective treatment strategies.
The study highlights the potential of nanobiosensors in advancing gynecological cancer diagnostics.

Keywords:

Electrochemical biosensor Graphene oxide Ovarian cancer Square wave voltammetry miRNA