Prediction strategy for screening functional Haloarchaea strains with qPCR assays
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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Published on: June 11, 2016
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Xinyu Hu1, Wenxiang Sun1, Meng Zhang2
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
Journal of Microbiological Methods
|September 8, 2024
Abstract:
As an extremophile resource, functional Haloarchaea strains are extremely time-consuming to screen. Here, taking the screening of low-salt-tolerant strains as an example, based on the qPCR assays that shortened time by 4-7 times and achieved 100 % accuracy, a universal strategy for rapid and accurate screening of functional Haloarchaea strains was established.

