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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jun 13, 2025

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

When do I ask for a DNA methylation array for primary brain tumor diagnosis?

Valeria Barresi¹, Pietro Luigi Poliani^2,3

¹Department of Diagnostics and Public Health, University of Verona, Verona.

Current Opinion in Oncology

|September 9, 2024

Summary

DNA methylation profiling enhances brain tumor diagnosis by providing reproducible and stable molecular data. This technique aids in classifying tumors and identifying new entities, improving patient management.

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Last Updated: Jun 13, 2025

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

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Published on: July 12, 2012

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Area of Science:

Neuro-oncology
Molecular diagnostics
Genomics

Background:

Brain tumor diagnosis is complex, often requiring integrated analysis of histology, molecular data, and clinical information.
Ambiguous histological or contradictory molecular features can challenge accurate brain tumor classification.
DNA methylation profiling offers a powerful tool to improve diagnostic and prognostic accuracy in neuro-oncology.

Purpose of the Study:

To provide diagnostic guidance on the utility of DNA methylation arrays for primary brain tumor diagnosis.
To highlight the role of DNA methylation profiling in overcoming diagnostic challenges in neuro-oncology.
To review the application of DNA methylation arrays in the context of current WHO classification guidelines.

Main Methods:

Review of large-scale DNA methylation profiling studies in brain tumors.
Analysis of the reproducibility and stability of DNA methylation profiles.
Evaluation of the classification power of DNA methylation profiling for various tumor types.

Main Results:

DNA methylation profiles of brain tumors are highly reproducible and stable.
DNA methylation profiling successfully classifies brain tumors and identifies novel entities.
This approach is particularly promising for heterogeneous tumor groups like IDH-wildtype gliomas and glioneuronal/embryonal tumors.

Conclusions:

Accurate brain tumor diagnosis requires integrating histological, molecular, clinical, and radiological data.
DNA methylation analysis significantly improves diagnostic accuracy beyond standard imaging and histology.
Implementing DNA methylation profiling has a substantial impact on patient management and treatment strategies.