JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

SOX11 as a potential prognostic biomarker in hepatocellular carcinoma linked to immune infiltration and ferroptosis

  • 0Department of Nephrology, the Second Medical Center of PLA General Hospital, National Clinical Research Center for Geriatric Diseases, Beijing 100853, China.
Chinese Journal of Cancer Research = Chung-Kuo Yen Cheng Yen Chiu +

|

September 9, 2024

|

September 9, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

SOX11 is elevated in hepatocellular carcinoma (HCC) and correlates with poor prognosis. Targeting SOX11 may offer a new therapeutic strategy for HCC patients.

Area Of Science

  • Oncology
  • Genetics
  • Bioinformatics

Background

  • SOX11 is implicated in various cancers, including hepatocellular carcinoma (HCC).
  • The precise role of SOX11 in HCC tumorigenesis remains unclear.
  • Understanding SOX11's function is crucial for developing targeted therapies.

Purpose Of The Study

  • To comprehensively investigate the role of SOX11 in HCC tumorgenesis.
  • To analyze SOX11 expression and its association with clinical features and patient outcomes in HCC.
  • To explore SOX11 as a potential diagnostic and prognostic biomarker for HCC.

Main Methods

  • Bioinformatics analysis of TCGA and GEO datasets for SOX11 expression.
  • Immunohistochemistry validation of SOX11 protein levels.
  • Functional enrichment analysis using Metascape and LinkedOmics.
  • Assessment of associations with immune infiltration, ferroptosis, and immune checkpoints.
  • In vitro studies on HCC cell lines (HepG2, HuH7) to evaluate proliferation and migration.

Main Results

  • SOX11 expression was significantly upregulated in HCC tumors compared to normal liver tissue.
  • SOX11 levels correlated with advanced HCC stage, higher histologic grade, and tumor status.
  • SOX11 independently predicted both overall and disease-specific survival in HCC patients.
  • SOX11 expression was associated with immune infiltration, ferroptosis, and immune checkpoint genes.
  • Reduced SOX11 expression in vitro led to decreased HCC cell proliferation and migration.

Conclusions

  • SOX11 serves as a potential diagnostic biomarker for HCC.
  • SOX11 is a significant prognostic indicator for HCC patient survival.
  • SOX11 represents a potential therapeutic target for HCC treatment.

Keywords: