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Updated: Jun 13, 2025
A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
Published on: September 11, 2020
Evaluation of MicroRNA 145 and MicroRNA 155 as Markers of Cardiovascular Risk in Chronic Kidney Disease
Amit Kumar1, G Priyadarshini1, Sreejith Parameswaran2
1Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, IND.
Abstract:
Background Chronic kidney disease (CKD) leads to a progressive decline in renal function, primarily due to deteriorating kidney structures. Vascular calcification is a key effect of CKD. MicroRNAs (miRNAs) play a significant role in the onset and progression of both cardiovascular illness and CKD. Aim The aim of this study was to compare biomarkers of endothelial dysfunction, 25-hydroxyvitamin D (25(OH)D), intact parathyroid hormone (iPTH), miRNA 155, and miRNA 145, in patients with CKD versus controls. Methods We recruited 60 patients with CKD and 60 controls. All participants underwent brachial artery flow-mediated dilatation (FMD). Asymmetric dimethylarginine (ADMA) levels were measured using ELISA. Levels of miRNA 145 and miRNA 155 were quantified using real-time polymerase chain reaction (PCR). Results Serum levels of miRNA 145, miRNA 155, 25(OH)D, and FMD were significantly lower in CKD patients compared to controls. Conversely, serum ADMA and iPTH levels were significantly higher in CKD patients. There was a significant negative association between miRNA 145, miRNA 155, FMD, and 25(OH)D with ADMA and iPTH. Additionally, miRNA 145, miRNA 155, FMD, and 25(OH)D showed a significant positive correlation with estimated glomerular filtration rate (eGFR) and with each other. Conclusion Lower levels of miRNA 145 and miRNA 155 and increased endothelial dysfunction correlate with CKD severity, suggesting an accelerated risk for cardiovascular disease (CVD).
Insights
Chronic kidney disease (CKD) is linked to lower levels of microRNA 145 and microRNA 155, alongside increased endothelial dysfunction. These findings suggest a higher cardiovascular disease risk in CKD patients.
Area of Science:
- Nephrology
- Cardiovascular Medicine
- Molecular Biology
Background:
- Chronic kidney disease (CKD) involves progressive renal function decline and structural damage.
- Vascular calcification is a significant complication of CKD.
- MicroRNAs (miRNAs) are implicated in the pathogenesis of cardiovascular disease and CKD.
Purpose of the Study:
- To compare biomarkers of endothelial dysfunction, 25-hydroxyvitamin D (25(OH)D), intact parathyroid hormone (iPTH), miRNA 155, and miRNA 145 between CKD patients and controls.
Main Methods:
- Sixty CKD patients and 60 controls were enrolled.
- Brachial artery flow-mediated dilatation (FMD) was assessed.
- Serum levels of ADMA, miRNA 145, and miRNA 155 were quantified.
Main Results:
- CKD patients exhibited lower serum levels of miRNA 145, miRNA 155, 25(OH)D, and FMD compared to controls.
- CKD patients showed significantly higher serum ADMA and iPTH levels.
- Negative associations were found between miRNA 145/155, FMD, 25(OH)D and ADMA/iPTH; positive correlations with eGFR.
Conclusions:
- Reduced levels of miRNA 145 and miRNA 155, coupled with heightened endothelial dysfunction, correlate with CKD severity.
- These factors indicate an elevated risk of cardiovascular disease in individuals with CKD.
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