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Updated: Jun 13, 2025
11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
12.9K
RNA binding protein with multiple splicing (RBPMS) promotes contractile phenotype splicing in human embryonic stem
Aishwarya G Jacob1,2, Ilias Moutsopoulos2, Alex Petchey2
1Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK.
Cardiovascular Research
|September 9, 2024
Summary
RNA Binding Protein with Multiple Splicing (RBPMS) drives smooth muscle-specific alternative pre-mRNA splicing (SM-AS) in human vascular smooth muscle cells (VSMCs). RBPMS promotes VSMC differentiation, impacting motility and proliferation.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Differentiated vascular smooth muscle cells (VSMCs) exhibit unique mRNA isoforms due to smooth muscle-specific alternative pre-mRNA splicing (SM-AS).
- RNA Binding Protein with Multiple Splicing (RBPMS) was previously identified as a key regulator of SM-AS in rat VSMCs.
Purpose of the Study:
- To investigate the role of RBPMS in SM-AS in human cells.
- To determine the impact of RBPMS on VSMC phenotypic properties.
Main Methods:
- Utilized human embryonic stem cell-derived VSMCs (hESC-VSMCs) as an experimental model.
- Over-expressed RBPMS in hESC-VSMCs to observe effects on splicing patterns.
- Performed in silico and experimental analyses to understand RBPMS splicing mechanisms.
- Assessed changes in VSMC motility and proliferation.
Main Results:
- Immature hESC-VSMCs showed low RBPMS levels and incomplete SM-AS patterns.
- RBPMS over-expression induced SM-AS patterns resembling those in contractile VSMCs.
- RBPMS directly binds to targets and cooperates with RBFOX2 for splicing.
- RBPMS altered hESC-VSMC motility and proliferation towards a differentiated state.
Conclusions:
- RBPMS plays a critical role in establishing the splicing program for the contractile phenotype in human VSMCs.
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