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Draft genome sequence of a spa non-typeable Staphylococcus aureus USA300 isolate causing complicated bacteremia
Emily A Felton1,2, Jesus Diaz Vera3, Ju Hee Katzman3
1Department of Molecular Biosciences, University of South Florida, Tampa, Florida, USA.
Microbiology Resource Announcements
|September 9, 2024
Summary
We present the genome sequence of a rare Spa-non-typeable Staphylococcus aureus strain, TGH1097. This USA300 isolate, recovered from bacteremia, offers insights into non-typeable strains.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Spa-typing is a key method for differentiating Staphylococcus aureus strains.
- A small percentage of S. aureus isolates (~1-2%) are considered Spa-non-typeable.
- Understanding these non-typeable strains is crucial for infection control.
Purpose of the Study:
- To present the draft genome sequence of a Spa-non-typeable Staphylococcus aureus strain.
- To characterize the genetic makeup of S. aureus TGH1097, a USA300 isolate.
- To contribute to the understanding of rare non-typeable S. aureus strains.
Main Methods:
- Whole-genome sequencing was performed on the S. aureus TGH1097 isolate.
- Bioinformatic analysis was used to assemble and annotate the draft genome.
- The isolate was identified as USA300 and originated from a complex bacteremia case.
Main Results:
- The draft genome sequence of Staphylococcus aureus TGH1097 was successfully generated.
- The strain belongs to the USA300 genetic background.
- The isolate was obtained from a patient with complex bacteremia.
Conclusions:
- The availability of this genome sequence provides a valuable resource for studying Spa-non-typeable Staphylococcus aureus.
- This data can aid in understanding the genetic basis and clinical significance of non-typeable strains.
- Further research on this strain may reveal unique adaptations or virulence factors.
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