Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Factors affecting microcin E492 production.

V de Lorenzo

    The Journal of Antibiotics
    |March 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Klebsiella pneumoniae RYC492 produces microcin E492. Culture medium composition significantly impacts antibiotic production, with gluconeogenic precursors yielding higher titers than glucose.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Interplay of the PtsN (EIIA(Ntr)) protein of Pseudomonas putida with its target sensor kinase KdpD.

    Environmental microbiology reports·2015
    Same author

    Recombinant bacteria for environmental release: what went wrong and what we have learnt from it.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2009
    Same author

    Improvement of recombinant protein yield by a combination of transcriptional amplification and stabilization of gene expression.

    Applied and environmental microbiology·2002
    Same author

    Towards the end of experimental (micro)biology?

    Environmental microbiology·2002
    Same author

    Deciphering the action of aromatic effectors on the prokaryotic enhancer-binding protein XylR: a structural model of its N-terminal domain.

    Environmental microbiology·2002
    Same author

    A la carte transcriptional regulators: unlocking responses of the prokaryotic enhancer-binding protein XylR to non-natural effectors.

    Molecular microbiology·2001
    Same journal

    Macrolide antibiotic action, resistance, and tolerance, and approaches to improve their activity against Gram-negative bacteria.

    The Journal of antibiotics·2026
    Same journal

    Activation of hamiformamide production in the thermotolerant fungus Hamigera avellanea triggered by co-culture with animal immune cells.

    The Journal of antibiotics·2026
    Same journal

    Biosynthesis and enzymology of azaindane natural products.

    The Journal of antibiotics·2026
    Same journal

    Inhibition of Proteus mirabilis growth through gallium-based disruption of iron and heme metabolism.

    The Journal of antibiotics·2026
    Same journal

    Artificial intelligence-based phenotypic screening using Aspergillus oryzae identified a novel antifungal RK-1205-I.

    The Journal of antibiotics·2026
    Same journal

    Catenulamide A, an acylated tripeptide containing 5-methoxy-L-kynurenine lactam from an actinomycete of the genus Catenuloplanes.

    The Journal of antibiotics·2026
    See all related articles

    Area of Science:

    • Microbiology
    • Biochemistry
    • Molecular Biology

    Background:

    • Klebsiella pneumoniae RYC492 produces the antibiotic polypeptide microcin E492.
    • Understanding factors influencing microcin E492 production is crucial for optimizing its yield.

    Purpose of the Study:

    • To investigate the effect of culture medium composition on microcin E492 production by Klebsiella pneumoniae RYC492.
    • To identify specific carbon and nitrogen sources that enhance microcin E492 titers.

    Main Methods:

    • Culturing Klebsiella pneumoniae RYC492 in various liquid media with different carbon and nitrogen sources.
    • Quantifying microcin E492 levels in culture supernatants using appropriate detection methods.
    • Assessing the impact of growth-limiting conditions (phosphate, nitrogen) and mitomycin C treatment.

    Related Experiment Videos

    Main Results:

    • Carbon sources significantly influenced microcin E492 levels; gluconeogenic precursors (lactate, citrate) resulted in 10-100 fold higher titers compared to glucose.
    • Glycerol and cAMP did not stimulate production, suggesting a mechanism distinct from typical glucose catabolite repression.
    • Phosphate or nitrogen limitation markedly decreased microcin production.
    • Mitomycin C treatment did not enhance microcin levels.

    Conclusions:

    • Culture medium composition, particularly the carbon source, is a critical determinant of microcin E492 production in Klebsiella pneumoniae.
    • Gluconeogenic precursors are superior to glucose for maximizing microcin E492 yield.
    • Microcin E492 production is sensitive to nutrient availability and may not be regulated by classical catabolite repression pathways.