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Investigation and quantification of composition variability in urinary stone analysis.
Binh Duy Le1,2, Kyung-Jin Oh3, Anh Tuan Le4,5
1Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju, Korea.
Investigative and Clinical Urology
|September 9, 2024
Summary
Urinary stone analysis shows significant composition variability between fragments. Careful sample mapping and individual fragment analysis can improve accuracy in clinical settings.
Area of Science:
- Urology
- Analytical Chemistry
- Materials Science
Background:
- Urinary stone (calculi) composition analysis is crucial for diagnosis and treatment.
- Variability in stone composition can arise from sampling methods.
- Accurate stone analysis informs patient management and preventative strategies.
Purpose of the Study:
- To quantify the variability in urinary stone composition based on sampling.
- To identify factors contributing to this variability.
- To propose methods for reducing analytical discrepancies.
Main Methods:
- Collected 1,135 urinary stone fragments from 149 patients.
- Analyzed fragment composition using Fourier transform infrared spectroscopy.
- Assessed variability between fragments within the same stone instance.
Main Results:
- Significant compositional variability was observed at the fragment level.
- 69.5% and 54.7% of multi-component stones showed significant variability at 20% and 30% thresholds, respectively.
- Variability correlated with fragment size and the number of components.
Conclusions:
- Urinary stone composition analysis exhibits substantial intra-stone variability.
- Fragment size and impurity influence compositional discrepancies.
- Improved sampling techniques, including fragment mapping and individual analysis, are recommended.
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