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Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

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Published on: September 19, 2014

Multi-layered silicone-based breast tissue phantom for multi-modal optical spectroscopy.

Subitcha Jayasankar¹, N Sujatha²

¹Indian Institute of Technology Madras, MSB 356, Department of Applied Mechanics, IIT Madras, Chennai, Tamil Nadu, 600036, INDIA.

Biomedical Physics & Engineering Express

|September 9, 2024

Summary

This summary is machine-generated.

This study developed a stable, multi-layered silicone phantom with tissue biomarkers to address challenges in spectroscopy-based diagnostics. The phantom aids in calibrating instruments for accurate tumor margin analysis using diffuse reflectance, fluorescence, and Raman spectroscopy.

Keywords:

Breast microcalcification Fluorescence spectroscopy Multi-modal phantoms Optical properties Raman spectroscopy Silicone phantoms Tissue phantoms

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Area of Science:

Biomedical Engineering
Optical Diagnostics
Materials Science

Background:

Biological tissue heterogeneity and ethical sourcing challenges impede spectroscopy-based diagnostic method development.
A stable, synthetic phantom mimicking tissue structure and biomarkers is crucial for calibration and standardization.

Purpose of the Study:

To develop a multi-layered silicone phantom incorporating multiple tissue biomarkers for spectroscopy testing and calibration.
To evaluate the phantom's utility for tumor margin analysis using multi-modal spectroscopy.

Main Methods:

Fabrication of a multi-layered silicone phantom.
Incorporation of hydroxyapatite to mimic microcalcifications.
Inclusion of Flavin Adenine Dinucleotide (FAD) and Nicotinamide Adenine Dinucleotide (NADH) for endogenous fluorescence.
Analysis using diffuse reflectance, fluorescence, and Raman spectroscopy.

Main Results:

The phantom successfully mimics multi-layered tissue structures and key biomarkers.
Observed intensity differences correlated with changes in simulated tumor layer depth and thickness.
Demonstrated suitability for instrument calibration and fiber-optic probe design.

Conclusions:

The developed synthetic phantom is a valuable tool for overcoming challenges in spectroscopy-based diagnostics.
The phantom facilitates instrument calibration and fiber-optic probe design for accurate tumor margin analysis.