Microvolumetric determination of thrombomodulin based on competitive immunoreaction using a portable glucometer
Peng Luo1, Yiyan Xie1, Xianhuan He1
1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, People's Republic of China.
Mikrochimica Acta
|September 9, 2024
Summary
A novel method uses nanocomposites and a portable glucometer to detect thrombomodulin (TM). This approach reduces reagent and sample volumes, offering a cost-effective and simpler alternative for TM determination.
Area of Science:
- Biotechnology
- Nanotechnology
- Biosensing
Background:
- Thrombomodulin (TM) determination is crucial in clinical diagnostics.
- Traditional methods for TM detection are often complex, costly, and require specialized equipment.
- There is a need for simplified, micro-volume assays for TM measurement.
Purpose of the Study:
- To develop a novel, cost-effective method for thrombomodulin (TM) determination.
- To reduce reagent and sample volumes in TM assays.
- To utilize a portable glucometer (PGM) for sensitive TM detection.
Main Methods:
- Preparation of two nanocomposites: TM protein-modified magnetic nanoparticles (MNPs-TM) and TM antibody-/glucose oxidase-modified gold nanoparticles (Ab-GNPs-GOx).
- Utilizing the competitive immunoreaction between TM and the prepared nanocomposites.
- Employing a portable glucometer (PGM) to measure glucose level changes catalyzed by the nanocomposite binding product.
Main Results:
- The TM-involved competitive immunoreaction inhibited glucose oxidation catalyzed by the nanocomposites.
- A linear relationship was observed between the logarithm of TM concentration (25 ng mL⁻¹ to 2.5 μg mL⁻¹) and the glucose content difference measured by PGM.
- Achieved a limit of detection of 5.7 ng mL⁻¹ for TM.
Conclusions:
- The developed method offers a sensitive and efficient approach for TM determination using micro-volumes.
- The use of nanocomposites and a PGM simplifies the assay procedure and reduces costs compared to conventional methods.
- This technique presents a viable alternative for point-of-care TM testing.
