Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Capillary ratchets activated by interfacial flows for versatile torque generation and microassembly.

Science advances·2026
Same author

IrTMes - a stable SABRE catalyst for the hyperpolarization of [1-<sup>13</sup>C]-pyruvate.

The Analyst·2026
Same author

In situ time-resolved motion of a tethered Pachnoda marginata, AI-correlated using μMRI and optical imaging.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same author

Minimalist optical system for achromatic imaging within extended field of view based on monolithic integrated meta-axicon cluster.

Light, science & applications·2026
Same author

Finite elements and moving asymptotes accelerate quantum optimal control-FEMMA.

The Journal of chemical physics·2026
Same author

Rapid RASER MRI.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Jun 13, 2025

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
07:12

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

10.0K

Development of a fully automated workstation for conducting routine SABRE hyperpolarization.

Jing Yang1, Ruodong Xin1, Sören Lehmkuhl1

  • 1Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), 76344, Eggenstein-Leopoldshafen, Germany.

Scientific Reports
|September 9, 2024
PubMed
Summary

A new automated workstation simplifies Spin Amplification by Buffer-Gas Exchange (SABRE) hyperpolarization for Nuclear Magnetic Resonance (NMR). This system enhances reproducibility and efficiency, making advanced NMR techniques more accessible for research.

More Related Videos

Optimization, Test and Diagnostics of Miniaturized Hall Thrusters
12:22

Optimization, Test and Diagnostics of Miniaturized Hall Thrusters

Published on: February 16, 2019

8.9K
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
08:59

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Published on: February 25, 2021

3.6K

Related Experiment Videos

Last Updated: Jun 13, 2025

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
07:12

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

10.0K
Optimization, Test and Diagnostics of Miniaturized Hall Thrusters
12:22

Optimization, Test and Diagnostics of Miniaturized Hall Thrusters

Published on: February 16, 2019

8.9K
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
08:59

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Published on: February 25, 2021

3.6K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Hyperpolarization Techniques
  • Chemical Instrumentation

Background:

  • Spin Amplification by Buffer-Gas Exchange (SABRE) offers a fast, simple, and low-cost method for enhancing NMR signals.
  • Manual SABRE execution leads to inconsistencies in reproducibility and efficiency.
  • Existing automated systems are complex and difficult to integrate into standard laboratory workflows.

Purpose of the Study:

  • To develop a fully automated laboratory workstation for SABRE hyperpolarization.
  • To facilitate SABRE experiments for various nuclei and polarization fields using a benchtop NMR spectrometer.
  • To overcome the limitations of manual SABRE and complex automated systems.

Main Methods:

  • Construction of a fully automated lab workstation integrated with a benchtop NMR spectrometer.
  • Implementation of routine SABRE experimental protocols for different nuclei (e.g., 13C).
  • Optimization of polarization transfer fields, measurement of polarization buildup rates, and decay times across various magnetic fields.
  • Iterative optimization of pulsed SABRE-SHEATH 13C pyruvate durations.

Main Results:

  • Demonstrated high reproducibility in consecutive SABRE hyperpolarizations (average standard deviation of 1.03%).
  • Successfully optimized 13C nuclei polarization with respect to the polarization transfer field.
  • Characterized polarization buildup rates and decay times over a wide range of magnetic fields.
  • Achieved optimized durations for pulsed SABRE-SHEATH 13C pyruvate.

Conclusions:

  • The developed SABRE workstation provides full automation, high reproducibility, and functional versatility.
  • It serves as a practical tool for routine SABRE hyperpolarization experiments.
  • The system offers a robust platform for high-throughput and reliable SABRE and X-SABRE hyperpolarization studies.