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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Structural basis for the type I-F Cas8-HNH system

Xuzichao Li1,2, Yanan Liu3, Jie Han1,4

  • 1Tianjin Institute of Immunology, State Key Laboratory of Experimental Hematology, International Joint Laboratory of Ocular Diseases (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China.

The EMBO Journal
|September 9, 2024
PubMed
Summary

A new CRISPR-Cas type I-F system uses a Cas8-HNH protein for precise DNA cleavage, unlike the typical Cas3 nuclease. Structural studies reveal how this system recognizes targets and activates DNA cutting for precise editing applications.

Keywords:
CRISPR-CasCas8-HNHGenome EditingHNH DomainType I-F System

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