JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Effect of ferric citrate on hippocampal iron accumulation and widespread molecular alterations associated with cognitive disorder in an ovariectomized mice model

  • 0College of Public Health, Zhengzhou University, Zhengzhou, Henan, China.
Cns Neuroscience & Therapeutics +

|

September 10, 2024

|

September 10, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Iron accumulation in ovariectomized mice impairs cognitive function and causes hippocampal damage. This study reveals iron metabolism protein alterations, providing insights into postmenopausal cognitive decline.

Area Of Science

  • Neuroscience
  • Endocrinology
  • Toxicology

Background

  • Cognitive impairment is increasingly prevalent in women, particularly postmenopause.
  • Limited research exists on the neurotoxic mechanisms of iron exposure in postmenopausal women.
  • Ovariectomy in mice serves as a model for postmenopausal changes.

Purpose Of The Study

  • To investigate the impact of iron accumulation on cognitive function in ovariectomized mice.
  • To elucidate the underlying mechanisms of iron-induced neurotoxicity in this model.
  • To provide a scientific basis for preventing cognitive dysfunction in postmenopausal women.

Main Methods

  • Ovariectomized (Ovx) mice were administered ferric citrate (FAC) at varying doses.
  • Cognitive and motor functions were assessed using behavioral tests.
  • Serum iron parameters, oxidative stress markers, hippocampal ultrastructure, and proteomic profiles were analyzed.

Main Results

  • Iron exposure significantly decreased motor and cognitive function in Ovx mice.
  • Histopathological changes were observed in the hippocampus following iron exposure.
  • Proteomic analysis revealed differential expression of hippocampal proteins, including increased transferrin receptor protein 1 (TFR1) and divalent metal transporter 1 (DMT1).

Conclusions

  • Iron exposure induces histopathological damage in the hippocampus of ovariectomized mice.
  • Altered hippocampal proteomics, particularly iron metabolism proteins, contributes to cognitive impairment.
  • Findings suggest a link between iron accumulation and postmenopausal cognitive dysfunction.

Keywords: