Related Experiment Video
Updated: Jun 13, 2025
Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
Published on: June 16, 2023
Decreased Serum CTRP3 level was associated with connective tissue diseases
Lisha Ma1, Jiangbo Lin2, Miaoli Shao1
1Department of Clinical Laboratory, The First People's Hospital of Wenling, Affiliated to Wenzhou Medical University, Taizhou, Zhejiang Province, China.
Insights
Serum levels of CTRP3 were significantly lower in patients with rheumatoid arthritis, lupus, Sjogren
Area of Science:
- Immunology
- Endocrinology
- Rheumatology
Background:
- Complement C1q tumor necrosis factor-related protein 3 (CTRP3) and 9 (CTRP9) are adipokines with diverse biological functions.
- Their association with connective tissue diseases (CTD) remains largely uninvestigated.
Purpose of the Study:
- To investigate the association between serum levels of CTRP3 and CTRP9 and various connective tissue diseases.
- To evaluate CTRP3 as a potential diagnostic marker for these conditions.
Main Methods:
- Serum CTRP3 and CTRP9 levels were measured using ELISA in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), primary Sjogren's syndrome (pSS), ankylosing spondylitis (AS), and undifferentiated connective tissue disease (UCTD), compared to healthy controls (HCs).
- Statistical analyses, including multivariate logistic regression and ROC curve analysis, were performed.
Main Results:
- Serum CTRP3 levels were significantly lower in patients with RA, SLE, pSS, and AS compared to HCs.
- No significant differences in serum CTRP9 levels were observed between patient groups and HCs.
- CTRP3 demonstrated potential as a diagnostic marker for RA, SLE, pSS, and AS, with AUC values ranging from 0.658 to 0.727.
Conclusions:
- Decreased serum CTRP3 levels are associated with rheumatoid arthritis, systemic lupus erythematosus, primary Sjogren's syndrome, and ankylosing spondylitis.
- CTRP3 may serve as a potential biomarker for these connective tissue diseases.
Objective:
Complement C1q tumor necrosis factor-related protein 3 (CTRP3) and 9 (CTRP9) are two of the most extensively studied adipokines, known for their diverse biological functions. However, it remains unclear whether serum levels of CTRP3 or CTRP9 are associated with connective tissue diseases (CTD).
Methods:
Serum CTRP3 and CTRP9 levels were measured by enzyme-linked immune sorbent assay (ELISA) and analyzed in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), primary Sjogren's syndrome (pSS), ankylosing spondylitis (AS), undifferentiated connective tissue disease (UCTD), as well as in healthy controls (HCs).
Results:
Serum CTRP3 levels were all significantly lower in patients with RA, SLE, pSS and AS compared with HCs. However, there were no significant differences in serum CTRP9 levels between patients with RA, SLE, pSS, or AS and HCs. In pSS patients, CTRP3 showed a weak correlation with blood glucose, creatinine, and urine acid in pSS patients, while no correlations were observed between serum CTRP3 levels and clinical or laboratory indices in RA, SLE or AS patients. Stable associations between CTRP3 and RA, SLE, pSS and AS were evaluated using multivariate logistics regression analysis. Receiver operating characteristic (ROC) curves were plotted to evaluated CTRP3 as a marker for RA, SLE, pSS and AS, yielding area under curve (AUC) values of 0.691, 0.727, 0.658 and 0.694, respectively.
Conclusion:
Decreased serum CTRP3 levels were associated with RA, SLE, pSS and AS.
More Related Videos
Related Concept Videos
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
01:25Blood Studies for Cardiovascular System III: Serum Lipid Profile
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...