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  11. Downregulation Of Lpar1 Promotes Invasive Behavior In Papillary Thyroid Carcinoma Cells

Downregulation of LPAR1 Promotes Invasive Behavior in Papillary Thyroid Carcinoma Cells

Zahra Bokaii Hosseini1, Fatemeh Rajabi2, Reza Morovatshoar3

  • 1Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.

Cancer Informatics
|September 10, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Lysophosphatidic acid receptor 1 (LPAR1) suppresses metastasis in papillary thyroid carcinoma (PTC) by regulating epithelial-mesenchymal transition (EMT). Lower LPAR1 expression and higher miR-221-5p indicate its potential as a diagnostic and therapeutic target for PTC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Lysophosphatidic acid receptor 1 (LPAR1) is a known biomarker in various cancers.
  • Its specific biological role in papillary thyroid carcinoma (PTC) has not been previously elucidated.

Purpose of the Study:

  • To investigate the function of LPAR1 in papillary thyroid carcinoma (PTC).
  • To determine if LPAR1 plays a role in epithelial-mesenchymal transition (EMT) and metastasis in PTC.
  • To explore LPAR1 as a potential biomarker and therapeutic target for PTC.

Main Methods:

  • Bioinformatics analysis of TCGA and GEO datasets to identify key regulators of EMT in PTC.
  • Protein-protein interaction (PPI) analysis and correlation with immune infiltrates.
  • Evaluation of LPAR1 expression using Gepia2, GTEx, and quantitative real-time polymerase chain reaction (qRT-PCR) in PTC cell lines.
Keywords:
LPAR1PTCbioinformaticscancer

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  • miRNA target gene prediction using multiMiR.

    • Main Results:

    • LPAR1 was identified as a key regulator of EMT in PTC, with 1081 genes upregulated and 544 downregulated compared to normal tissue.
    • LPAR1 expression correlated positively with CD4+ T cells, macrophages, neutrophils, and myeloid dendritic cells, but negatively with B cells.
    • miR-221-5p was predicted to target LPAR1, and experimental data showed LPAR1 was significantly under-expressed in a PTC cell line compared to a normal thyroid cell line (P < .01).

    Conclusions:

    • LPAR1 suppresses metastasis and is associated with EMT in PTC.
    • Decreased LPAR1 expression and increased miR-221-5p in PTC suggest LPAR1's role in disease progression.
    • LPAR1 holds potential as a diagnostic biomarker, prognostic indicator, and therapeutic target for EMT in PTC.
    diagnosis