Cigarette smoking prolongs inflammation associated with influenza infection and delays its clearance in mice

Epidemiological studies have shown that smoking is associated with increased incidence of severe viral infections leading to hospitalization. Moreover, studies in experimental models have identified impaired antiviral responses and altered inflammatory responses, yet it is unclear how the effects of smoke exposure and influenza A infection interact and how this varies over the course of infection. We hypothesized that smoking would exacerbate innate immune responses against influenza. To test this, female BALB/c mice were exposed to cigarette smoke or air twice a day for 24-28 days and (mock) infected with H3N2 influenza A on day 21 while smoking continued. About 3 and 7 days after infection, changes in immune cell populations, the transcriptome, and viral clearance in lung tissue were analyzed. After influenza A infection, smoke-exposed mice lost significantly more weight than air-exposed controls, indicating that smoking resulted in more severe disease. Immune cell and lung tissue transcriptome analysis revealed that neutrophil infiltration was prolonged and macrophage activation dysregulated after infection of smoke-exposed mice compared with air-exposed controls. Expression of genes in IL-6 and interferon pathways was similarly longer active. In parallel, we observed slower clearance of influenza virus in smoke-exposed mice after infection compared with air-exposed controls, indicating ineffective antiviral responses. Altogether, the data from our mouse model indicate that cigarette smoke exposure prolongs innate immune responses against influenza A. The results from this study help to explain the susceptibility of current smokers to severe influenza A disease.NEW & NOTEWORTHY In this study, we describe how cigarette smoke exposure modulates immune responses against influenza in mice over time. Using a unique model that continues smoke exposure after infection, we demonstrate that inflammation is prolonged and viral clearance is delayed. This clinically relevant model for smokers that contract influenza is well positioned to investigate interactions between smoke and influenza infection.