Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Globular and Fibrous Proteins02:21

Globular and Fibrous Proteins

43.5K
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
43.5K
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

3.5K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.5K
Fibrous Proteins00:55

Fibrous Proteins

2.1K
Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...
2.1K
Protein Complex Assembly02:41

Protein Complex Assembly

10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding chemical reactions through multimedia resolution data artistic educational tool (MRDAET).

Protein science : a publication of the Protein Society·2026
Same author

EnzymeMiner 2.0: advancing automated enzyme discovery with expansive sequence mining and smart property analysis.

Nucleic acids research·2026
Same author

Taurine inhibits apolipoprotein E4 aggregation.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Experimentally validated deep learning control of protein aggregation.

Communications chemistry·2026
Same author

Structural insights into the evolution of alpha/beta-hydrolase fold luciferases.

International journal of biological macromolecules·2026
Same author

The effect of salidroside, an active component of Rhodiola rosea, on the metabolic activity of rat and human cytochromes P450 in preclinical studies.

Pharmacological reports : PR·2026
Same journal

MesoSplats: Texture Synthesis with Gaussian Splatting.

IEEE transactions on visualization and computer graphics·2026
Same journal

GLLA: A Unified Force-Directed Graph Layout Framework Supporting Local Adjustments.

IEEE transactions on visualization and computer graphics·2026
Same journal

Multi-Perception Crowd: Learning to combine entity and implicit perception for diverse crowd simulation.

IEEE transactions on visualization and computer graphics·2026
Same journal

Hiding in Plain Sight: Camouflaging Real-world Objects.

IEEE transactions on visualization and computer graphics·2026
Same journal

RTF2Mesh: Restricted Tangent Face Based Mesh Compression With Neural Displacement Fields.

IEEE transactions on visualization and computer graphics·2026
Same journal

Practical Occluder Generation for Mobile Games.

IEEE transactions on visualization and computer graphics·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2025

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.3K

Visual Support for the Loop Grafting Workflow on Proteins.

Filip Opaleny, Pavol Ulbrich, Joan Planas-Iglesias

    IEEE Transactions on Visualization and Computer Graphics
    |September 10, 2024
    PubMed
    Summary
    This summary is machine-generated.

    Protein engineers use interactive visualizations for loop grafting, a method to transfer protein functions. This computational approach aids in designing novel protein structures for in vitro experiments.

    More Related Videos

    A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
    08:12

    A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

    Published on: July 11, 2017

    7.3K
    A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
    10:52

    A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

    Published on: October 30, 2012

    10.7K

    Related Experiment Videos

    Last Updated: Jun 13, 2025

    Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
    07:08

    Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

    Published on: July 14, 2015

    7.3K
    A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
    08:12

    A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

    Published on: July 11, 2017

    7.3K
    A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
    10:52

    A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

    Published on: October 30, 2012

    10.7K

    Area of Science:

    • Biochemistry
    • Structural Biology
    • Protein Engineering

    Background:

    • Protein function is often determined by specific regions known as loops.
    • Loop grafting is a key technique for protein redesign and functional transfer.
    • Current methods require sophisticated analysis of loop characteristics.

    Purpose of the Study:

    • To develop interactive visualizations supporting the entire loop grafting pipeline.
    • To provide visual tools for protein engineers to design novel protein functions.
    • To facilitate the computational rational design of proteins and their loops.

    Main Methods:

    • Designed a workflow with interactive 2D and 3D visualizations for loop analysis.
    • Implemented a phased approach reflecting loop grafting pipeline steps.
    • Integrated visual representations linked to a 3D protein view for candidate selection.

    Main Results:

    • Successfully visualized and supported all stages of the loop grafting process.
    • Enabled users to identify and select candidate loops for grafting through interactive exploration.
    • Demonstrated the computational design of new protein structures via in-silico loop insertion.

    Conclusions:

    • Interactive visualizations significantly enhance the protein engineering process for loop grafting.
    • The developed tool facilitates the rational design of proteins with altered functions.
    • Visual support accelerates the creation of novel protein structures for experimental validation.