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HEMATOLOGIC AND PLASMA BIOCHEMICAL REFERENCE VALUES FOR CAPTIVE LEOPARD SHARKS (TRIAKIS SEMIFASCIATA)
Amanda J Arndt1, Meghan M Holst2, Jill V Spangenberg3
1Aquarium of the Bay, San Francisco, CA 94133, USA, Amanda.joy.arndt@gmail.com.
Summary
This study establishes crucial blood reference values for captive leopard sharks (Triakis semifasciata), aiding in their health assessment. These findings improve veterinary care for this popular aquarium species.
Area of Science:
- Marine Biology
- Comparative Physiology
- Veterinary Medicine
Background:
- Leopard sharks (Triakis semifasciata) are popular in public aquariums.
- Established blood reference values are essential for assessing the health of captive elasmobranchs.
- No prior reference values existed for this species.
Purpose of the Study:
- To characterize blood reference values for captive leopard sharks.
- To provide essential data for improved veterinary diagnostics and care.
- To establish a baseline for future physiological studies.
Main Methods:
- Analyzed blood samples from 21 captive leopard sharks.
- Collected blood during routine annual health examinations.
- Measured hematocrit and plasma biochemistry values using standard laboratory analysis.
Main Results:
- Established minimum-maximum ranges for hematocrit, glucose, electrolytes (sodium, potassium, chloride), liver enzymes (AST), muscle enzymes (CK), minerals (calcium, phosphorus), total protein, and total CO2.
- Identified specific ranges for key blood parameters in T. semifasciata.
- Provided comprehensive hematological and biochemical data.
Conclusions:
- The established blood reference values enhance understanding of captive leopard shark physiology.
- These data are vital for improving the veterinary care and health monitoring of leopard sharks in aquariums.
- Further research should validate diagnostic tools for broader application in elasmobranch medicine.

